Effect of Solanum surattense on mitochondrial enzymes in diabetic rats and in vitro glucose uptake activity in L6 myotubes.
Loading...
Date
2015-10
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Background: S. surattense is widely used in Siddha medicine for various ailments. Objective: The aim was to evaluate the
impact of alcoholic leaf‑extract of S. surattense on mitochondrial enzymes in streptozotocin (STZ) induced diabetic rats
and to study the in vitro muscle glucose uptake activity on L6 myotubes. Materials and Methods: The male albino Wistar
rats were randomly divided into five groups of six animals each. Diabetes was induced by intraperitoneal injection of STZ
(40 mg/kg body weight). After being confirmed the diabetic rats were treated with alcoholic leaf‑extract of S. surattense
(100 mg/kg body weight) for 45 days. The biochemical estimations (liver mitochondrial enzymes, antioxidants, thiobarbituric
acid reactive substances [TBARS]) and histopathological studies were performed. Further, the in vitro muscle glucose
uptake activity in L6 myotubes and messenger RNA (mRNA) expression of glucose transporter‑4 (GLUT‑4) was performed.
Results: In diabetic rats, the activities of liver mitochondrial enzymes were found to be significantly lowered. The mitochondrial
TBARS level increased, whereas the activities/level of enzymatic and non‑enzymatic antioxidants decreased in diabetic rats.
Administration of S. surattense to diabetic rats significantly reversed the above parameters toward normalcy. Furthermore
in diabetic rats, the histopathological studies showed growth of adipose tissue and shrinkage of islets in the pancreas,
liver showed fatty change with mild inflammation of portal triad, and kidney showed messangial capillary proliferation of
glomeruli and fatty infiltration of tubules. Treatment with S. surattense brought back these changes to near normalcy.
The extract was analyzed for in vitro muscle glucose uptake activity in L6 myotubes and mRNA expression of GLUT‑4 by
semi‑quantitative reverse transcriptase‑polymerase chain reaction. One nano gram per millilitre of S. surattense leaf‑extract
gave 115% glucose uptake on L6 myotubes. It also showed elevated levels of GLUT‑4 mRNA transcripts, when compared
with control cells. Conclusion: These studies strongly support the anti‑diabetic nature of S. surattense.
Description
Keywords
Glucose uptake, glucose transporter‑4, mitochondrial enzymes, Solanum surattense, streptozotocin, thiobarbituric acid reactive substances
Citation
Sridevi Muruhan, Kalairarasi Pannerselvam, Pugalendi Kodukkur Viswanathan. Effect of Solanum surattense on mitochondrial enzymes in diabetic rats and in vitro glucose uptake activity in L6 myotubes.Journal of Ayurveda and Integrative Medicine. 2015 Oct-Dec; 6(4): 259-267.