Functional analysis of ATP-binding cassette transporter of Streptomyces coelicolor.
Date
2014-07
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Abstract
ATP- binding cassette (ABC) transporters are characterized as multi drug resistant transporters utilizing ATP
hydrolysis. The SCO5113 (GeneID: 1100554) encoding a multidrug resistance ABC transporter in Streptomyces
coelicolor was expressed in E.coli BL21 (DE3) plysS, using pET 21 a(+). The expression of his – tag fused
SCO5113 at 65 kDa under 0.1 M IPTG in 6 h was detected on SDS-PAGE after purification using affinity
chromatography. The E. coli BL21 (DE3) plysS harboring SCO5113 was used to test for the antibiotic resistance,
using disc diffussion method. The results showed the resistance of E. coli BL21 (DE3) plysS harboring SCO5113
to five kinds of tested antibiotics such as cephalothin (1mg/ml), kanamycin (1mg/ml), ampicillin (10mg/ml),
erythromycin (10mg/ml), and chloramphenicol (1mg/ml). The study reported the function of the SCO5113 gene
of Streptomyces coelicolor A3(2).
Description
Keywords
ABC transporter, antibiotic resistance, functional analysis, Streptomyces coelicolor
Citation
Nguyen Tu, Nguyen Huu. Functional analysis of ATP-binding cassette transporter of Streptomyces coelicolor. Journal of Applied Pharmaceutical Science. 2014 July; 4(7): 50-53.