High-risk human papillomavirus in Turkish patients with clinically suspicious cervical lesions analyzed by multiplex-PCR

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dc.contributor.authorGorur, Leventen_US
dc.contributor.authorDolanbay, Mehmeten_US
dc.contributor.authorOzturk, Figenen_US
dc.contributor.authorCanoz, Ozlemen_US
dc.contributor.authorDonmez-Altuntas, Hamiyeten_US
dc.date.accessioned2023-08-19T04:52:30Z
dc.date.available2023-08-19T04:52:30Z
dc.date.issued2022-12
dc.description.abstractBackground & objectives: Human papillomavirus (HPV) infection is known to be the main cause of cervical cancer. This study aimed to determine the prevalence of high-risk HPV genotypes in smear specimens taken from women who had normal or abnormal cytology using a multiplex PCR method. Methods: The study included 270 women aged between 19 and 69 yr with or without suspicious cervical abnormalities. A Pap smear sample from each patient was cytologically examined, and HPV typing was performed using a multiplex fluorescent PCR method. Those who were high-risk HPV positive and had a normal or abnormal cytology were further evaluated by colposcopy and biopsy. Results: The total HPV positivity was 43 per cent (116/270). HPV positivity in the patients with an abnormal cytology was 77 per cent (33/43), whereas it was only 37 per cent (83/227) in women with normal cytology, which showed a significant difference (P<0.05). HPV positivity was also related to the age group when all the subjects were considered (P<0.05), and the highest prevalence of HPV infection was in the 30-39 yr age group. High-risk HPV types 16, 18, 31, 35, 51 and 56 were more common in the normal cytology patients, whereas high-risk HPV types 16, 31, 35, 45, 58 and 68 were commonly found in the abnormal cytology patients. Interpretation & conclusions: The determination of high-risk HPV genotypes in women with clinically suspicious cervical lesions should be conducted during an annual follow-up, irrespective of a normal or abnormal cytology by the age of 30 years or above.en_US
dc.identifier.affiliationsDepartments ofen_US
dc.identifier.affiliationsMedical Biologyen_US
dc.identifier.affiliationsGynecology & Obstetricsen_US
dc.identifier.affiliationsMedical Pathology, Faculty of Medical, Erciyes University, Kayseri, Turkeyen_US
dc.identifier.citationGorur Levent, Dolanbay Mehmet, Ozturk Figen, Canoz Ozlem, Donmez-Altuntas Hamiyet. High-risk human papillomavirus in Turkish patients with clinically suspicious cervical lesions analyzed by multiplex-PCR. Indian Journal of Medical Research. 2022 Dec; 156(6): 786-791en_US
dc.identifier.issn0971-5916
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/223707
dc.languageenen_US
dc.publisherWolters Kluwer – Medknowen_US
dc.relation.issuenumber6en_US
dc.relation.volume156en_US
dc.source.urihttps://doi.org/10.4103/ijmr.IJMR_2335_20en_US
dc.subjectAbnormal cytologyen_US
dc.subjectcervical canceren_US
dc.subjecthigh-risk human papillomavirusen_US
dc.subjectmultiplex fluorescent PCRen_US
dc.subjectThinPrep Pap testen_US
dc.subjectTurkish womenen_US
dc.titleHigh-risk human papillomavirus in Turkish patients with clinically suspicious cervical lesions analyzed by multiplex-PCRen_US
dc.typeJournal Articleen_US
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