Enzyme linked immunosorbent assay & indirect fluorescence assay for rapid diagnosis of Bacteroides fragilis infections.

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dc.contributor.authorAyyagari, Aen_US
dc.contributor.authorKhanna, Ten_US
dc.contributor.authorDevi, Sen_US
dc.date.accessioned1992-01-01en_US
dc.date.accessioned2009-05-27T06:52:23Z
dc.date.available1992-01-01en_US
dc.date.available2009-05-27T06:52:23Z
dc.date.issued1992-01-01en_US
dc.description.abstractAntibodies for B. fragilis (NCTC 9343) were detected in sera of 121 patients and 37 controls using four methods viz., enzyme linked immunosorbent assay (ELISA), indirect fluorescence test (JFA), countercurrent immunoelectrophoresis (CIE) and indirect haemagglutination test (IHA). Of 121 patients, 57 were culture positive for B. fragilis, 38 positive for anaerobes other than B. fragilis and 26 were negative for anaerobes. In the B. fragilis culture positive group, antibodies to B. fragilis were positive in 82.5, 84.2, 85.90 and 91.2 per cent patients by CIE, ELISA, IHA and IFA respectively. In B. fragilis positive patients IFA was more sensitive than IHA, which for other groups IHA was found to be more sensitive than IFA. When all groups were taken together IHA was found more sensitive than IFA. ELISA and IFA tests are recommended for rapid serological diagnosis of B. fragilis infections, where facilities for these tests are not available, CIE and IHA could be done. Cross reactivity with other Gram negative anaerobic and aerobic bacteria should be kept in mind since seropositivity varied for B. fragilis (82-91%). In infections with microbes other than B. fragilis seropositivity varied between 23.7 to 63.2 per cent and in patients having cultures sterile or positive for other organisms seropositivity was 30.8 to 42.3 per cent. This nonspecificity could be due to other antigens that cross react between B. fragilis and other anaerobes and aerobes or the use of an antigen lacking high purity.en_US
dc.description.affiliationDepartment of Medical Microbiology, Postgraduate Institute of Medical Education & Research Chandigarh.en_US
dc.identifier.citationAyyagari A, Khanna T, Devi S. Enzyme linked immunosorbent assay & indirect fluorescence assay for rapid diagnosis of Bacteroides fragilis infections. Indian Journal of Medical Research. 1992 Jan; 95(): 34-40en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/18298
dc.language.isoengen_US
dc.source.urihttps://icmr.nic.in/ijmr/ijmr.htmen_US
dc.subject.meshAntibodies, Bacterial --blooden_US
dc.subject.meshBacteroides Infections --diagnosisen_US
dc.subject.meshBacteroides fragilis --immunologyen_US
dc.subject.meshCounterimmunoelectrophoresisen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshFemaleen_US
dc.subject.meshFluorescent Antibody Techniqueen_US
dc.subject.meshHemagglutination Testsen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.titleEnzyme linked immunosorbent assay & indirect fluorescence assay for rapid diagnosis of Bacteroides fragilis infections.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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