Evaluation of lipid peroxidation product, nitrite and antioxidant levels in newly diagnosed and two months follow-up patients with pulmonary tuberculosis.

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dc.contributor.authorLamsal, Madhaben_US
dc.contributor.authorGautam, Narayanen_US
dc.contributor.authorBhatta, Narendraen_US
dc.contributor.authorToora, Bishamber Dassen_US
dc.contributor.authorBhattacharya, Shymal Kumaren_US
dc.contributor.authorBaral, Nirmalen_US
dc.date.accessioned2009-05-27T16:54:22Z
dc.date.available2009-05-27T16:54:22Z
dc.date.issued2007-07-22en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractThis case-control study followed by a longitudinal cohort study was undertaken to evaluate the level of lipid peroxidation product malondialdehyde (MDA) and nitrite as an indirect measurement of nitric oxide vis-à-vis the levels of antioxidants vitamin C and vitamin E in pulmonary tuberculosis. Fifty-six sputum smear-positive cases of pulmonary tuberculosis based on Ziehl-Neelsen (ZN) staining and 50 healthy controls without any systemic disease were included in this study. Thirty-five cases were longitudinally followed up with standard antituberculosis chemotherapy (ATT) for two months. Serum levels of malondiadehyde (MDA), nitrite, and plasma levels of vitamins C and E were measured. The mean serum MDA level was significantly higher (8.1 +/- 1.61 nmoles/ml) in PTB patients before commencement of ATT as compared to healthy controls (3.45 +/- 1.7 nmoles/ml) (p=0.0001) and decreased significantly after 2 months of ATT (3.84 +/- 1.28 nmoles/ml) (p=0.0001). The mean serum nitrite level (47.19 +/- 18.44 micromol/l) was significantly elevated before ATT compared to healthy controls (32.89 +/- 11.94 micromoles/l) and decreased significantly after 2 months of ATT (27.71 +/- 11.97 micromoles/l) (p=0.0001). The mean plasma levels of vitamins C (0.88 +/- 0.33 mg/dl) and E (0.79 +/- 0.24 mg/dl) in PTB patients before commencement of ATT were lower than healthy controls (1.42 +/- 0.38 mg/dl) and (1.35 +/- 0.35 mg/dl), respectively (p=0.001). There was a significant increase in vitamin C levels after 2 months of ATT (1.19 +/- 0.40 mg/dl) compared to before ATT (0.83 +/- 0.31 mg/dl) (p=0.0001), but no significant change in mean plasma vitamin E level before and after 2 months on ATT was found. Elevated malondialdehyde and nitrite levels with concomitant depressed vitamin C and E levels are indicative of lipid peroxidation and oxidative stress. The decrease in levels of malondialdehyde and nitrite with subsequent increase in vitamin C levels after two months of follow-up indicate a good response to treatment with standard ATT. Hence, the extent of oxidative stress in PTB can be evaluated by analyzing lipid peroxidation product, antioxidant and nitric oxide levels.en_US
dc.description.affiliationDepartment of Biochemistry, B. P. Koirala Institute of Health Sciences, Dharan, Nepal. madhablamsal@yahoo.co.uken_US
dc.identifier.citationLamsal M, Gautam N, Bhatta N, Toora BD, Bhattacharya SK, Baral N. Evaluation of lipid peroxidation product, nitrite and antioxidant levels in newly diagnosed and two months follow-up patients with pulmonary tuberculosis. The Southeast Asian Journal of Tropical Medicine and Public Health. 2007 Jul; 38(4): 695-703en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/36276
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/2007_38_4/15-3858.pdfen_US
dc.subject.meshAdulten_US
dc.subject.meshAntioxidants --analysisen_US
dc.subject.meshAscorbic Acid --blooden_US
dc.subject.meshCohort Studiesen_US
dc.subject.meshFemaleen_US
dc.subject.meshFollow-Up Studiesen_US
dc.subject.meshHumansen_US
dc.subject.meshLipid Peroxidation --physiologyen_US
dc.subject.meshLongitudinal Studiesen_US
dc.subject.meshMaleen_US
dc.subject.meshMalondialdehyde --analysisen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshNepalen_US
dc.subject.meshNitric Oxide --analysisen_US
dc.subject.meshSputumen_US
dc.subject.meshTuberculosis, Pulmonary --metabolismen_US
dc.subject.meshVitamin E --blooden_US
dc.titleEvaluation of lipid peroxidation product, nitrite and antioxidant levels in newly diagnosed and two months follow-up patients with pulmonary tuberculosis.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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