Enhancement of ?-amylase production in pelleted Aspergillus tamarii through optimization for desizing of cotton fabric

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dc.contributor.authorR., Mohanen_US
dc.contributor.authorR., Subramanianen_US
dc.contributor.authorS., Muthiahen_US
dc.contributor.authorS., Natarajanen_US
dc.date.accessioned2020-11-18T10:16:20Z
dc.date.available2020-11-18T10:16:20Z
dc.date.issued2019-09
dc.description.abstractAim: The present study was carried out to enhance the production of α-amylase by pelleted Aspergillus tamarii through optimization of various media compositions and cultivation conditions using shake flask and bioreactor experiments. Methodology: Shake flask experiments were conducted to study the effect of pH, temperature and starch concentration using Response Surface Methodology (RSM) and other parameters, namely, nitrogen source, inoculum size and incubation days using single variable optimization technique for the pelleted growth of microorganism and amylase production. Scale up study was carried out for the assessment of results obtained from shake flask experiments using a laboratory scale bioreactor. In the bioreactor study, parameters, namely, pH control, agitation and aeration were considered. Results: Maximum amylase production using pelleted Aspergillus tamarii was achieved at initial pH 6.7, temperature 30.5 °C, 0.5% w/v starch, 0.1% w/v urea, 1.5% v/v inoculum size and 4 days of fermentation in the shake flask experiments. Filamentous growth was observed when the concentration of starch used was above 2%. The specific enzyme activity increased to 2.77 fold after partial purification. When enzyme was used for desizing cotton fabric, it produced 90% efficiency. The scale-up experiments revealed initial pH 6.7, agitation 300 rpm and aeration 1 vvm as the conditions suitable for pelleted growth, as well as to achieve maximum amylase production. Interpretation: The results indicate pelleted growth of Aspergillus tamarii and in turn achievement of maximum amylase activity depends on media composition and fermentation conditions used at the time of enzyme production. Efficient desizing of cotton fabric by amylase showed its effectiveness towards hydrolysis of starch and converting it to soluble products for easy removal.en_US
dc.identifier.affiliationsDepartment of Textile Technology, Anna University, Chennai–600 025, Indiaen_US
dc.identifier.affiliationsCenter for Biotechnology, Anna University, Chennai–600 025, Indiaen_US
dc.identifier.affiliationsBiological Material Laboratory, CSIR-Central Leather Research Institute, Chennai–600 020, Indiaen_US
dc.identifier.affiliationsDepartment of Textile Technology, Anna University, Chennai–600 025, Indiaen_US
dc.identifier.citationR. Mohan, R. Subramanian, S. Muthiah, S. Natarajan. Enhancement of ?-amylase production in pelleted Aspergillus tamarii through optimization for desizing of cotton fabric. Journal of Environmental Biology . 2019 Sep; 40(5): 1084-1093en_US
dc.identifier.issn0254-8704
dc.identifier.issn2394-0379
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/214631
dc.languageenen_US
dc.publisherTriveni Enterprisesen_US
dc.relation.issuenumber5en_US
dc.relation.volume40en_US
dc.source.urihttps://www.jeb.co.in/journal_issues/201909_sep19/paper_15.pdfen_US
dc.subjectAspergillus tamariien_US
dc.subjectCotton fabricen_US
dc.subjectDesizingen_US
dc.subjectOptimizationen_US
dc.subjectα-amylaseen_US
dc.titleEnhancement of ?-amylase production in pelleted Aspergillus tamarii through optimization for desizing of cotton fabricen_US
dc.typeJournal Articleen_US
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