Bioreduction of silver nanoparticles from aerial parts of Euphorbia hirta L. (EH-ET) and its potent anticancer activities against neuroblastoma cell lines

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dc.contributor.authorSelvam, Pen_US
dc.contributor.authorVijayakumar, Ten_US
dc.contributor.authorWadhwani, Ashishen_US
dc.contributor.authorMuthulakshmi, Len_US
dc.date.accessioned2020-01-02T06:23:21Z
dc.date.available2020-01-02T06:23:21Z
dc.date.issued2019-04
dc.description.abstractEuphorbia hirta L. (Family: Euphorbiaceae) is a versatile medicinal plant and enriched with novel bioactive molecules and possess broad-spectrum pharmacological actions. Present work is aiming to synthesis and characterize of silver nanoparticles (AgNPs) by bioreduction method an using ethanolic extract of aerial parts of Euphorbia hirta L. (EH-ET). The synthesized AgNPs observed by a color change of source solution (as AgNPs) and further confirmed by the UV-Visible spectroscopic technique. The AgNPs synthesized were characterized by Scanning Electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR) and Zeta potential analysis. The synthesized AgNPs are polydispersed and some places it’s agglomerated. The particle size EH-ET silver nanoparticles were analyzed using Beckman coulter particle size analyzer (DelsaTM Nano common). The average size of the particles size noted in 2.9-206.3 nm. Anticancer activity of EH-ET Silver nanoparticles was tested against neuroblastoma cells (SH-SY5Y) and breast cancer cells (SH-SY5Y) and cytotoxicity were tested in vero cells by MTT assay. The preliminary confirmation of the synthesized AgNPs by the present method was made by the appearance of reddish brown color and the visible absorption peak at 429.5 nm. SEM image revealed that AgNPs synthesized were spherical in shape and silver nanoparticles were in the size range of 2.9 to 206.3 nm. FT-IR spectra showed the peaks corresponding to functional groups C=O, -C=C and -OH, which actively participated in bio-reduction and subsequent stabilization reactions in the synthesis of AgNPs. The obtained nanoparticles showed promising anticancer activity against neuroblastoma cells (SH-SY5Yc) and breast cancer cells (MCF-7) with IC50 values of 29.85 and 335 µg/mL, respectively. Whereas, the nanoparticles did not show any activity against vero cell lines. The synthesized silver nanoparticles using an ethanolic extract of Euphorbia hirta L. would be helpful for the preparation of potent cytotoxicity agents to destroy cancer cells.en_US
dc.identifier.affiliationsInternational Research Centre, University, Krishnankoil -626 126, Tamil Nadu, Indiaen_US
dc.identifier.affiliationsDepartment of Biotechnology, Kalasalingam University, Krishnankoil -626 126, Tamil Nadu, Indiaen_US
dc.identifier.affiliationsDepartment of Pharmaceutical Biotechnology, JSS College of Pharmacy, Jagadguru Sri Shivarathreeshwara University, Ooty -643 001, Tamil Nadu, Indiaen_US
dc.identifier.citationSelvam P, Vijayakumar T, Wadhwani Ashish, Muthulakshmi L. Bioreduction of silver nanoparticles from aerial parts of Euphorbia hirta L. (EH-ET) and its potent anticancer activities against neuroblastoma cell lines. Indian Journal of Biochemistry and Biophysics. 2019 Apr; 56(2): 132-136en_US
dc.identifier.issn0975-0959
dc.identifier.issn0301-1208
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/190035
dc.languageenen_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.relation.issuenumber2en_US
dc.relation.volume56en_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/46917en_US
dc.titleBioreduction of silver nanoparticles from aerial parts of Euphorbia hirta L. (EH-ET) and its potent anticancer activities against neuroblastoma cell linesen_US
dc.typeJournal Articleen_US
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