Development of lyophilization cycle for direct thrombin Inhibitor and influence of excipients and process parameter during the cycle.

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dc.contributor.authorPanda, Sunil Kumar
dc.contributor.authorDinda, Subas Chandra
dc.date.accessioned2014-07-24T09:43:59Z
dc.date.available2014-07-24T09:43:59Z
dc.date.issued2013-04
dc.description.abstractThe objective of this study was to develop stable lyophilized product of Bivalirudin, a direct thrombin inhibitor with good reconsti-tution time, % yield of good vials and cake quality. The % loss of crystallinity was determined by DSC for any crystallographic changes of the bulking agent after freeze drying. The glass transition temperature of 5% w/v of Bivalirudin, 3% w/v of mannitol and 0.03% of sodium acetate was approximately -31°C and the collapse temperature was approximately -28°C. Tubular vials were found to withstand the thermal transition during freeze drying. Water content was inversely proportional to the primary drying set point. Reconstitution time was inversely proportional to annealing temperature and vacuum set point. The % yield of good vials and cake quality was directly proportional to annealing time and primary drying set point. The % loss of crystallinity by DSC was independ-ent of all factors and directly proportional to annealing time. Drying at -10°C results in transition of β form of mannitol, whereas drying at -7.5°C and -5°C results in α and δ form of mannitol respectively. On annealing at -7°C for 54 minutes and drying at -6.5°C at 215mT lyophilized product with less than 2% water content, reconstitution time less than 10 seconds, with high yield of more than 95% yield of good vials with best cake quality was obtained.en_US
dc.identifier.citationPanda Sunil Kumar, Dinda Subas Chandra. Development of lyophilization cycle for direct thrombin Inhibitor and influence of excipients and process parameter during the cycle. International Current Pharmaceutical Journal. 2014 Apr 3(5): 259-264.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/153087
dc.language.isoenen_US
dc.source.urihttps://www.icpjonline.com/documents/Vol3Issue5/01Abstract.htmen_US
dc.subjectDirect thrombin inhibitorsen_US
dc.subject% loss of crystallinityen_US
dc.subjectreconstitution timeen_US
dc.subjectlyophilizeden_US
dc.subjectannealing temperatureen_US
dc.titleDevelopment of lyophilization cycle for direct thrombin Inhibitor and influence of excipients and process parameter during the cycle.en_US
dc.typeArticleen_US
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