A factor in a wild isolated Neurospora crassa strain enables a chromosome segment duplication to suppress repeat-induced point mutation.
dc.contributor.author | Ramakrishnan, Mukund | |
dc.contributor.author | Sowjanya, T Naga | |
dc.contributor.author | Raj, Kranthi B | |
dc.contributor.author | Kasbekar, Durgadas P | |
dc.date.accessioned | 2015-08-08T09:11:25Z | |
dc.date.available | 2015-08-08T09:11:25Z | |
dc.date.issued | 2011-12 | |
dc.description.abstract | Repeat-induced point mutation (RIP) is a sexual stage-specific mutational process of Neurospora crassa and other fungi that alters duplicated DNA sequences. Previous studies from our laboratory showed that chromosome segment duplications (Dps) longer than ~300 kbp can dominantly suppress RIP, presumably by titration of the RIP machinery, and that although Dps <200 kbp did not individually suppress RIP, they could do so in homozygous and multiply heterozygous crosses, provided the sum of the duplicated DNA exceeds ~300 kbp. Here we demonstrate suppression of RIP in a subset of progeny carrying the normally sub-threshold 154 kbp Dp(R2394) from a cross of T(R2394) to the wild isolated Carrefour Mme. Gras strain (CMG). Thus, the CMG strain contains a factor that together with Dp(R2394) produces a synthetic RIP suppressor phenotype. It is possible that the factor is a cryptic Dp that together with Dp(R2394) can exceed the size threshold for titration of the RIP machinery and thereby causes RIP suppression. | en_US |
dc.identifier.citation | Ramakrishnan Mukund, Sowjanya T Naga, Raj Kranthi B, Kasbekar Durgadas P. A factor in a wild isolated Neurospora crassa strain enables a chromosome segment duplication to suppress repeat-induced point mutation. Journal of Biosciences. 2011 Dec; 36 (5): 817-821. | en_US |
dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/161615 | |
dc.language.iso | en | en_US |
dc.source.uri | https://www.ias.ac.in/jbiosci/dec2011/817.pdf | en_US |
dc.subject | Chromosome segment duplications | en_US |
dc.subject | dominant suppressor of RIP | en_US |
dc.subject | genome defense in fungi | en_US |
dc.title | A factor in a wild isolated Neurospora crassa strain enables a chromosome segment duplication to suppress repeat-induced point mutation. | en_US |
dc.type | Article | en_US |