High prevalence of cagA positive Vac A s1m1 Helicobacter pylori strains isolated from patients suffering from various gastroduodenal diseases in Guwahati, Assam, India

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dc.contributor.authorMahant, Sen_US
dc.contributor.authorSingh, Sen_US
dc.contributor.authorDutta, Sen_US
dc.contributor.authorSharma, Nen_US
dc.contributor.authorDas, Pen_US
dc.date.accessioned2025-06-19T05:55:13Z
dc.date.available2025-06-19T05:55:13Z
dc.date.issued2025-03
dc.description.abstractAim and Background: Genotyping of H. pylori strains was identified on formalin?fixed paraffin wax?embedded tissue (FFPE) sections and correlated with severity grades of gastric mucosal pathologies in biopsies from upper gastrointestinal (UGI) tract from Guwahati, Assam. Materials and Methods: In total, 168 gastric biopsies collected from patients with UGI symptoms underwent histological evaluation as per the updated Sydney system. Result: H. pylori?like organisms were identified with Warthin and Starry stain, and virulent genes were amplified using polymerase chain reaction (PCR) from genomic DNA extracted from FFPE sections by using QIAamp® DNA FFPE Tissue Kit. Histological examination identified H. pylori?like organisms in 100 biopsies, of which 96 were urease + ve. The prevalence of H. pylori infection was high in age groups 71–80 (88.8%) as compared to other age groups, and it was higher in females (78.9%) when compared to males. The prevalence of virulent genes in biopsies was 88.5% cagA and vacA s1m1, 31.2% iceA1, 32.2% iceA2, and 85.2% babA2. The histological parameters mononuclear cell infiltrate (P = 0.04) and atrophy (P = 0.03), showed statistically significant association with iceA2 and intestinal metaplasia with cagA (P = 0.01) vacAs1m1 (P = 0.01) and babA (P = 0.02) genotypes. Gastric erosion due to H. pylori infection and atrophy showed a significant association. A high bacterial density score was seen with the virulent genotypes. Conclusion: Our work reports for the first time a high prevalence (88.5%) of H. pylori cagA vacA s1m1 genotype in Guwahati, Assam. Association of gastric atrophy and intestinal metaplasia was seen with virulent genotypes. Results show the effectiveness of the FFPE kit for DNA extraction in remote areas where transportation and storage of biopsies are otherwise difficult.en_US
dc.identifier.affiliationsCentre for Medical Biotechnology, Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradeshen_US
dc.identifier.affiliationsCentre for Medical Biotechnology, Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradeshen_US
dc.identifier.affiliationsAmity Institute of Biotechnology, Guwahati Medical College, Guwahati, Assamen_US
dc.identifier.affiliationsDepartment of Pathology, All India Institute of Medical Science, New Delhien_US
dc.identifier.affiliationsDepartment of Pathology, All India Institute of Medical Science, New Delhien_US
dc.identifier.affiliationsNational Institute of Cholera and Enteric Diseases, Kolkata, West Bengalen_US
dc.identifier.affiliationsDepartment of Gastroenterology, Yashoda Super Specialty Hospital, Ghaziabad, Uttar Pradesh, Indiaen_US
dc.identifier.affiliationsCentre for Medical Biotechnology, Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradeshen_US
dc.identifier.citationMahant S, Singh S, Dutta S, Sharma N, Das P. High prevalence of cagA positive Vac A s1m1 Helicobacter pylori strains isolated from patients suffering from various gastroduodenal diseases in Guwahati, Assam, India. Indian Journal of Pathology & Microbiology. 2025 Mar; 68(1): 51-60en_US
dc.identifier.issn0377-4929
dc.identifier.issn0974-5130
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/249965
dc.languageenen_US
dc.publisherWolters Kluwer - Medknowen_US
dc.relation.issuenumber1en_US
dc.relation.volume68en_US
dc.source.urihttps://doi.org/10.4103/ijpm.ijpm_1002_23en_US
dc.subjectFormalin?fixed paraffin?embedded biopsiesen_US
dc.subjectHelicobacter pylorien_US
dc.subjecthistologyen_US
dc.subjectvirulent genesen_US
dc.titleHigh prevalence of cagA positive Vac A s1m1 Helicobacter pylori strains isolated from patients suffering from various gastroduodenal diseases in Guwahati, Assam, Indiaen_US
dc.typeJournal Articleen_US
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