Molecular Detection of Plasmid-Mediated Quinolone Resistance Genes (qnrA and aac(6?)-Ib-cr) in Drug Resistant Escherichia coli, Sudan

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dc.contributor.authorTageldin, Men_US
dc.contributor.authorBabier, A. Men_US
dc.contributor.authorElhasan, O. Aen_US
dc.contributor.authorYousif, Men_US
dc.contributor.authorAhemir, S. Yen_US
dc.contributor.authorHussien, H. Men_US
dc.contributor.authorYousif, S. Men_US
dc.contributor.authorIbrahim, S. Oen_US
dc.contributor.authorMirgani, H. Oen_US
dc.contributor.authorMohamed, H. Een_US
dc.contributor.authorGamar, T. Aen_US
dc.contributor.authorAhmed, E. A.en_US
dc.date.accessioned2023-06-27T06:26:59Z
dc.date.available2023-06-27T06:26:59Z
dc.date.issued2023-06
dc.description.abstractBackground: The quinolone group, a synthetic antimicrobial, is widely used worldwide to treat many diseases, including those caused by Gram-negative bacteria. Escherichia coli and others are among the bacteria that produce quinolone resistance genes (qnr) such as qnrA and aac(6?)-Ib-cr. Objective: The present study aimed to the isolate Escherichia coli from patients attending some Hospitals in Wad Medani city, identification of drug resistance patterns and detection of the frequency of quinolones resistance genes; qnrA and aac(6?)-Ib-cr among isolated strains. Methods: A cross-sectional descriptive, hospital-based study included 119 Escherichia coli strains was conducted. A designed questionnaire used for demographic data collection and the attitude toward antimicrobials usage. Clinical specimens were processed for aerobic bacterial isolation and identification. Antimicrobial sensitivity performed by Kirby Bauer disc diffusion technique according to the CLSI guidelines. Presence of qnrA and aac(6?)-Ib-cr genes was assessed by multiplex PCR. Results: Most strains of Escherichia coli originated from urine 53.8% (64/119) and wounds 42.9% (51/119) specimens. Meropenem had the best effect against tested strains with susceptibility of 85% (101/119). Multiplex PCR assay, using specific primers, demonstrated that 41.2% (49/119) and 37.8% (45/119) of isolated Escherichia coli possessed qnrA and aac(6?)-Ib-cr genes respectively. Conclusion: The high rate of qnrA and aac (6)-Ib-cr genes among Escherichia coli necessitate the usage of molecular tools in detecting the genetic determinants of drug resistance microorganisms in countries such as Sudan.en_US
dc.identifier.affiliationsDepartment of Medical Microbiology, Faculty of Medical Laboratory Sciences, University of Gezira, Wad Medani, Sudanen_US
dc.identifier.affiliationsNational Cancer Institute, University of Gezira, Wad Medani, Sudanen_US
dc.identifier.affiliationsFaculty of Pharmacy, University of Gezira, Wad Medani, Sudanen_US
dc.identifier.affiliationsNational Blood Bank, Ministry of Health, Khartoum, Sudanen_US
dc.identifier.affiliationsDepartment of Medical Parasitology, Faculty of Medical Laboratory Sciences, University of Science and Technology, Khartoum, Sudanen_US
dc.identifier.citationTageldin M, Babier A. M, Elhasan O. A, Yousif M, Ahemir S. Y, Hussien, H. M, Yousif S. M, Ibrahim S. O, Mirgani, H. O, Mohamed H. E, Gamar T. A, Ahmed E. A.. Molecular Detection of Plasmid-Mediated Quinolone Resistance Genes (qnrA and aac(6?)-Ib-cr) in Drug Resistant Escherichia coli, Sudan. Microbiology Research Journal International. 2023 Jun; 33(4): 14-21en_US
dc.identifier.issn2456-7043
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/217205
dc.languageenen_US
dc.publisherSciencedomain Internationalen_US
dc.relation.issuenumber4en_US
dc.relation.volume33en_US
dc.source.urihttps://doi.org/10.9734/mrji/2023/v33i41375en_US
dc.subjectEscherichia colien_US
dc.subjectquinolone resistancen_US
dc.subjectqnrA aac(6?)-Ib-cren_US
dc.subjectmultiplex-PCRen_US
dc.subjectSudanen_US
dc.titleMolecular Detection of Plasmid-Mediated Quinolone Resistance Genes (qnrA and aac(6?)-Ib-cr) in Drug Resistant Escherichia coli, Sudanen_US
dc.typeJournal Articleen_US
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