Effect of the human follicle-stimulating hormone-binding inhibitor and its N-terminal fragment on follicle-stimulating hormone-induced progesterone secretion by granulosa cells in vitro.

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dc.contributor.authorWadia, Perinaaz Ren_US
dc.contributor.authorMahale, Smita Den_US
dc.contributor.authorNandedkar, Tarala Den_US
dc.date.accessioned2007-09-24en_US
dc.date.accessioned2009-06-01T14:11:08Z
dc.date.available2007-09-24en_US
dc.date.available2009-06-01T14:11:08Z
dc.date.issued2007-09-24en_US
dc.description.abstractIntrafollicular factors play an important role in folliculogenesis. The follicle-stimulating hormone (FSH)-binding inhibitor (FSHBI), purified by our laboratory from human ovarian follicular fluid, has been shown to suppress ovulation and induce follicular atresia/apoptosis in mice as well as impair fertility in marmosets, the New World monkeys. The octapeptide, a peptide corresponding to the N-terminal region of human FSHBI (hFSHBI), has been synthesized and also shows FSHBI activity in vitro. In the present study, we have attempted to identify the mechanism of action of the peptide in granulosa cell cultures. Rat granulosa cell cultures were treated with varying concentrations of the octapeptide or partially purified hFSHBI (gel chromatography fraction hGF 2) in the presence or absence of human FSH (hFSH) and the amount of progesterone (P;4) secreted in the culture supernatants after 3 h/48 h was estimated. Both hGF2 and the octapeptide failed to alter basal levels as well as 8-bromo cAMP-induced P;4 production, while FSH-induced P 4 secretion was inhibited in a dose-dependent manner. These studies reveal that the octapeptide, a fragment of FSHBI, and the native protein have similar activity in vitro and both compounds alter FSH action at the receptor level upstream of cAMP formation.en_US
dc.description.affiliationDepartment of Cell Biology, National Institute for Research in Reproductive Health, JM Street, Parel, Mumbai 400012, India.en_US
dc.identifier.citationWadia PR, Mahale SD, Nandedkar TD. Effect of the human follicle-stimulating hormone-binding inhibitor and its N-terminal fragment on follicle-stimulating hormone-induced progesterone secretion by granulosa cells in vitro. Journal of Biosciences. 2007 Sep; 32(6): 1185-94en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/110648
dc.language.isoengen_US
dc.source.urihttps://www.ias.ac.in/jbiosci/index.htmlen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCarrier Proteins --physiologyen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCyclic AMP --metabolismen_US
dc.subject.meshDown-Regulation --physiologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshFollicle Stimulating Hormone --antagonists & inhibitorsen_US
dc.subject.meshGlycopeptides --physiologyen_US
dc.subject.meshGranulosa Cells --metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshIntercellular Signaling Peptides and Proteins --physiologyen_US
dc.subject.meshPeptide Fragments --physiologyen_US
dc.subject.meshProgesterone --antagonists & inhibitorsen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshReceptors, FSH --antagonists & inhibitorsen_US
dc.titleEffect of the human follicle-stimulating hormone-binding inhibitor and its N-terminal fragment on follicle-stimulating hormone-induced progesterone secretion by granulosa cells in vitro.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, N.I.H., Extramuralen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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