Derivative Spectrophotometric Methods for the Determination of Bendamustine Hydrochloride.
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Date
2012-11
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Abstract
Bendamustine hydrochloride is used to treat chronic lymphocytic leukemia. It kills the existing cancer
cells and limits the growth of new cancer cells. Three simple, rapid and sensitive spectrophotometric
methods were developed for the determination of Bendamustine hydrochloride in phosphate buffer (pH
8.0) (Method A) and boric buffer (pH 9.0) (Method B). Method C is a difference spectroscopy
technique in which the amplitude was chosen for the analytical calculations. Bendamustine
hydrochloride obeys Beer-Lambert’s law over the concentration range 1-40 μg/ml, 0.1-40 μg/ml and 5-
40 μg/ml with regression equations y = 0.003x + 0.001(r² = 0.998) y = 0.0027x + 0.0005 (r² = 0.999),
and y = 0.0034x + 0.006 (r² = 0.994) for Method A, B and C respectively. The methods were validated
as per ICH guidelines and can be applied for the determination of Bendamustine hydrochloride in
pharmaceutical formulations.
Description
Keywords
Bendamustine hydrochloride, Derivative spectroscopy, Difference spectroscopy, Validation
Citation
Annapurna M Mathrusri, Pavani S, Anusha S, Mahanti Harika. Derivative Spectrophotometric Methods for the Determination of Bendamustine Hydrochloride. Journal of Applied Pharmaceutical Science. 2012 Nov; 2(11): 139-142.