Downregulation of apolipoprotein A-IV in plasma & impaired reverse cholesterol transport in individuals with recent acts of deliberate self-harm

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dc.contributor.authorMathew, Ben_US
dc.contributor.authorSrinivasan, Ken_US
dc.contributor.authorPradeep, Jen_US
dc.contributor.authorThomas, Ten_US
dc.contributor.authorMurthy, SKen_US
dc.contributor.authorMandal, AKen_US
dc.date.accessioned2020-04-10T01:44:22Z
dc.date.available2020-04-10T01:44:22Z
dc.date.issued2019-10
dc.description.abstractBackground & objectives: The major limiting factor in the prevention of suicide is the limited knowledge on molecular insights in individuals at risk. Identification of peripheral protein markers which can classify individuals at high-risk of suicide might aid in early diagnosis and effective medical intervention. The aim of the present study was, therefore, to analyze the differential regulation of plasma proteins in individuals with deliberate self-harm compared to controls. Methods: Using two-dimensional gel electrophoresis coupled with matrix-assisted laser desorption-ionization mass spectrometry, differentially expressed plasma proteins were identified in study participants with deliberate self-harm compared to age- and gender-matched controls. The finding was validated using mass spectrometry-based isotope-labelled relative quantification and Western blot analysis in a new set of individuals with deliberate self-harm and controls. Results: The plasma proteomic analysis showed that apolipoprotein A-IV (Apo A-IV ) was downregulated by 2.63-fold (confidence interval: 1.52-4.54) in individuals with deliberate self-harm (n=10) compared to matched controls, which was consistent in mass spectrometry-based relative quantification and Western blot analysis performed in an independent set of individuals with deliberate self-harm (n=18). In addition, plasma levels of total cholesterol, esterified cholesterol and high-density lipoprotein (HDL) were observed to be significantly lower individuals with deliberate self-harm compared to controls. Interpretation & conclusions: Apo A-IV, which plays a crucial role in the esterification of free cholesterol, was found to be downregulated with concomitantly decreased levels of HDL, esterified cholesterol and total cholesterol in individuals with deliberate self-harm compared to matched controls. The present findings might provide a link between the differential regulation of plasma proteins and the previously reported results on altered cholesterol levels in individuals with deliberate self-harm.en_US
dc.identifier.affiliationsDivision of Molecular Medicine, Clinical Proteomics Unit, St. John's Research Institute, St. John's National Academy of Health Sciences, Bengaluru, Karnataka, Indiaen_US
dc.identifier.affiliationsDepartment of Psychiatry, St. Johns Medical College, St. John's National Academy of Health Sciences, Bengaluru, Karnataka, Indiaen_US
dc.identifier.affiliationsDepartment of Biostatistics, St. Johns Medical College, St. John's National Academy of Health Sciences, Bengaluru, Karnataka, Indiaen_US
dc.identifier.affiliationsDepartment of Emergency Medicine, St. Johns Medical College, St. John's National Academy of Health Sciences, Bengaluru, Karnataka, Indiaen_US
dc.identifier.citationMathew B, Srinivasan K, Pradeep J, Thomas T, Murthy SK, Mandal AK. Downregulation of apolipoprotein A-IV in plasma & impaired reverse cholesterol transport in individuals with recent acts of deliberate self-harm. Indian Journal of Medical Research. 2019 Oct; 150(4): 365-375en_US
dc.identifier.issn0971-5916
dc.identifier.issn0975-9174
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/196051
dc.languageenen_US
dc.publisherIndian Council of Medical Researchen_US
dc.relation.issuenumber4en_US
dc.relation.volume150en_US
dc.source.urihttps://dx.doi.org/10.4103/ijmr.IJMR_1842_17en_US
dc.subjectApolipoprotein A-IVen_US
dc.subjectdeliberate self-harmen_US
dc.subjectesterified cholesterolen_US
dc.subjectmass spectrometryen_US
dc.subjectsuicideen_US
dc.subjecttwo-dimensional gel electrophoresisen_US
dc.titleDownregulation of apolipoprotein A-IV in plasma & impaired reverse cholesterol transport in individuals with recent acts of deliberate self-harmen_US
dc.typeJournal Articleen_US
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