High-risk human papillomavirus, tumor suppressor protein p53 and mitomycin-C in invasive squamous cell carcinoma cervix.

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dc.contributor.authorRajaram, Sen_US
dc.contributor.authorGupta, Gen_US
dc.contributor.authorAgarwal, Sen_US
dc.contributor.authorGoel, Nen_US
dc.contributor.authorSingh, K Cen_US
dc.date.accessioned2009-05-28T06:14:24Z
dc.date.available2009-05-28T06:14:24Z
dc.date.issued2006-10-29en_US
dc.description.abstractBACKGROUND: Clinical data relating to human papillomavirus (HPV) infection and p53 status in cervical cancer has been sparse and confusing. AIM: To evaluate high-risk HPV and expression of tumor suppressor protein p53 in squamous cell carcinoma of cervix and to assess response to mitomycin-C in neo-adjuvant chemotherapy. SETTING AND DESIGN: Teaching College Hospital; Gynecologic Oncology Unit and Department of Pathology. Prospective, randomized. MATERIALS AND METHODS: Expression of p53 protein was assessed, using immunohistochemistry with mouse monoclonal antibody in 30 consecutive patients undergoing radical hysterectomy or admitted for neo-adjuvant chemotherapy. Human papillomavirus DNA (HPV DNA) was assessed using hybrid capture II technology. Patients eligible for chemotherapy were randomized into vincristine, bleomycin and cisplatin (VBP) group and VBP with mitomycin C group. STATISTICAL ANALYSIS: Chi-square test, one-way ANOVA, Pearson's correlation; Mann-Whitney, McNemar and Fischer's exact tests were used for statistical analysis. RESULTS: All patients with cancer cervix were positive for high-risk HPV DNA having relative light units/cut off values ranging from 3.4-2389.21 (P value = 0.006). High viral load of high risk HPV DNA was seen in advanced stages (P = 0.05) and an association of viral load with tumor volume was also seen (r = 0.361, P = 0.05). Analysis of p53 protein in cervical carcinoma patient showed expression in 50% of cancer specimens (P value < 0.001). McNemar's and Fischer's exact test showed no change in p53 status post-chemotherapy; however 66% of stage II B patients in VBP-M group became operable. CONCLUSION: High-risk HPV was universally present in all cases of cancer cervix and viral load was associated with stage and tumor volume while p53 protein was expressed in 50% of cases suggesting deregulation. More studies using mitomycin-C in cervical cancer treatment protocols are needed.en_US
dc.description.affiliationDepartment of Obstetrics and Gynaecology, Guru Teg Bahadur Hospital, University College of Medical Sciences, Delhi - 110 095, India. shalini_rajaram@rediffmail.comen_US
dc.identifier.citationRajaram S, Gupta G, Agarwal S, Goel N, Singh KC. High-risk human papillomavirus, tumor suppressor protein p53 and mitomycin-C in invasive squamous cell carcinoma cervix. Indian Journal of Cancer. 2006 Oct-Dec; 43(4): 156-62en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/49526
dc.language.isoengen_US
dc.source.urihttps://www.indianjcancer.comen_US
dc.subject.meshAdolescenten_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAged, 80 and overen_US
dc.subject.meshAntineoplastic Combined Chemotherapy Protocols --therapeutic useen_US
dc.subject.meshBleomycin --administration & dosageen_US
dc.subject.meshCarcinoma, Squamous Cell --drug therapyen_US
dc.subject.meshChemotherapy, Adjuvanten_US
dc.subject.meshChilden_US
dc.subject.meshCisplatin --administration & dosageen_US
dc.subject.meshDNA, Viral --geneticsen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoenzyme Techniquesen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshMitomycin --administration & dosageen_US
dc.subject.meshNeoadjuvant Therapyen_US
dc.subject.meshPapillomaviridaeen_US
dc.subject.meshPapillomavirus Infections --complicationsen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshProspective Studiesen_US
dc.subject.meshTumor Suppressor Protein p53 --metabolismen_US
dc.subject.meshUterine Cervical Neoplasms --drug therapyen_US
dc.subject.meshVincristine --administration & dosageen_US
dc.subject.meshViral Loaden_US
dc.titleHigh-risk human papillomavirus, tumor suppressor protein p53 and mitomycin-C in invasive squamous cell carcinoma cervix.en_US
dc.typeJournal Articleen_US
dc.typeRandomized Controlled Trialen_US
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