Comparative studies on IFAT, ELISA & DAT for serodiagnosis of visceral leishmaniasis in Bangladesh.

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dc.contributor.authorAlam, M Jen_US
dc.contributor.authorRahman, K Men_US
dc.contributor.authorAsna, S Men_US
dc.contributor.authorMuazzam, Nen_US
dc.contributor.authorAhmed, Ien_US
dc.contributor.authorChowdhury, M Zen_US
dc.date.accessioned1996-04-01en_US
dc.date.accessioned2009-05-27T03:46:52Z
dc.date.available1996-04-01en_US
dc.date.available2009-05-27T03:46:52Z
dc.date.issued1996-04-01en_US
dc.descriptionBangladesh Medical Research Council Bulletin.en_US
dc.description.abstractThe purpose of the study was to estimate the specificity and sensitivity of different serological methods for the diagnosis of visceral leishmaniasis in Bangladesh. Blood samples from 155 suspected kala-azar patients together with 80 sick subjects and 50 healthy subjects from the endemic areas were collected. Out of the 155 suspected kala-azar patients, bone marrow were collected from 126 patients. All bone marrow samples were examined by direct microscopy. 92 bone marrow samples were also examined by culture method. Blood samples were examined by various serological tests. Out of 126 marrow samples, LD bodies were present by microscopy in 77 (61.1%) cases and out of 92 marrow samples, cultures for LD bodies were positive in 33 (35.9%) cases. All the three serological tests (IFAT, ELISA & DAT) were positive in all parasitologically positive kala-azar patients. They were also positive in seven (15.5%) out of 45 parasitologically negative cases and 10 (34.4%) out of remaining 29 cases in whom bone marrow samples were not available. Thus the serological tests proved to be simple, non-invasive, highly sensitive and specific methods for the diagnosis of visceral leishmaniasis. DAT is the simplest of these serological tests, although these tests did not differ in sensitivity and specificity.en_US
dc.description.affiliationSher-e-Bangla Medical College, Barisal.en_US
dc.identifier.citationAlam MJ, Rahman KM, Asna SM, Muazzam N, Ahmed I, Chowdhury MZ. Comparative studies on IFAT, ELISA & DAT for serodiagnosis of visceral leishmaniasis in Bangladesh. Bangladesh Medical Research Council Bulletin. 1996 Apr; 22(1): 27-32en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/112
dc.language.isoengen_US
dc.source.urihttps://www.banglajol.info/index.php/BMRCB/indexen_US
dc.subject.meshAgglutination Testsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBangladeshen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshFluorescent Antibody Technique, Indirecten_US
dc.subject.meshHumansen_US
dc.subject.meshLeishmania donovanien_US
dc.subject.meshLeishmaniasis, Visceral --diagnosisen_US
dc.subject.meshSensitivity and Specificityen_US
dc.subject.meshSerologic Testsen_US
dc.titleComparative studies on IFAT, ELISA & DAT for serodiagnosis of visceral leishmaniasis in Bangladesh.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
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