In vitro cytotoxicity MTT assay in Vero, HepG2 and MCF -7 cell lines study of Marine Yeast.
| dc.contributor.author | Senthilraja, P | |
| dc.contributor.author | Kathiresan, K | |
| dc.date.accessioned | 2015-07-06T09:52:23Z | |
| dc.date.available | 2015-07-06T09:52:23Z | |
| dc.date.issued | 2015-03 | |
| dc.description.abstract | Marine yeasts isolated from coastal mangrove ecosystem namely Candida albicans, Kuraishia capsulate and Sacchromyces cerevisae were screened for the cytotoxicity against a non-transformed Vero (African green monkey kidney normal cell line) and two cancer cell lines human breast carcinoma cells (HepG2), human breast carcinoma cells (MCF -7) in different concentrations (1000 to 1.953 μg/ml). Lower doses enhanced the viability of the cultured cells, MTT assay .higher doses decreased viability of the cells by 50% or more. MTT assay was used to measure the cell proliferation and survival. Amongst three yeast strains, Sacchromyces cerevisae showed more than 80% cell viability in Vero cell lines and were studied for further cytotoxicity against HepG2, MCF -7 cell lines respectively. | en_US |
| dc.identifier.citation | Senthilraja P, Kathiresan K. In vitro cytotoxicity MTT assay in Vero, HepG2 and MCF -7 cell lines study of Marine Yeast. Journal of Applied Pharmaceutical Science. 2015 Mar; 5(3): 80-84. | en_US |
| dc.identifier.issn | 2231-3354 | |
| dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/159142 | |
| dc.language.iso | en | en_US |
| dc.source.uri | https://www.japsonline.com/counter.php?aid=1457 | en_US |
| dc.subject | Yeast | en_US |
| dc.subject | cancer | en_US |
| dc.subject | MTT assay | en_US |
| dc.subject | Cell culture | en_US |
| dc.subject | Vero | en_US |
| dc.subject | HepG2 | en_US |
| dc.subject | MCF-7 | en_US |
| dc.title | In vitro cytotoxicity MTT assay in Vero, HepG2 and MCF -7 cell lines study of Marine Yeast. | en_US |
| dc.type | Article | en_US |