miR-7160 inhibits gastric cancer cell proliferation and metastasis by silencing SIX1
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Date
2024-08
Journal Title
Journal ISSN
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Publisher
The Indian Academy of Sciences
Abstract
Upregulation of homeoprotein SIX1 in gastric cancer (GC) is related to tumour proliferation and invasion. MicroRNA-7160 (miR-7160) is a homeoprotein SIX1-targeting miRNA that downregulates miR-7160, leading to cancer development. Total gastric cancer samples were collected from six patients, and relative expression levels of SIX1 mRNA and miRNAs were analysed by qRT-PCR. To evaluate the regulation of SIX1 by miR-7160, pGL3-SIX1-mut, pGL3-SIX1, and miR-7160 mimics transfected into cells using lipofec- tamine 2000. After transfection, proliferation and apoptosis in cultured cells were assessed using the nuclear TUNEL staining and CCK8 reagent, respectively. We demonstrated that the downregulation of miR-7160 in human gastric cancer cells is related to the upregulation of SIX1 mRNA. In gastric cancer cell lines, miR-7160 overexpression could downregulate the expression and inhibit cancer cell proliferation and growth in vitro. However, overexpression of miR-7160 did not increase gastric cancer cell apoptosis. In vitro downregulation of SIX1 decreased vimentin, N-cadherin, and other EMT-related gene expression and increased E-cadherin expression. In brief, miR-7160, by targeting SIX1, inhibits gastric cancer proliferation and cell growth in vitro, which provides an idea for introducing a new treatment option for gastric cancer.
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Keywords
SIX1 gene, miR-7160, cell growth, cell proliferation, gastric cancer.
Citation
MENG MENG, GUAN GUOXIN, LIU XINGMING, SUN WEI, CUI XINYE, FU SAIYA, LUO FUWEN . miR-7160 inhibits gastric cancer cell proliferation and metastasis by silencing SIX1. Journal of Genetics . 2024 Aug; 103: 1-10