Comparison of circulating DNA in malignant neoplasia from diverse locations: Investigating a diagnostic role

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dc.contributor.authorKumari, Sen_US
dc.contributor.authorMishra, Sen_US
dc.contributor.authorHusain, Nen_US
dc.contributor.authorVerma, Ten_US
dc.contributor.authorTiwari, Ven_US
dc.contributor.authorKaif, Men_US
dc.contributor.authorAgarwal, Aen_US
dc.contributor.authorRastogi, Men_US
dc.contributor.authorShukla, Sen_US
dc.contributor.authorSonkar, AA.en_US
dc.date.accessioned2023-08-10T07:32:13Z
dc.date.available2023-08-10T07:32:13Z
dc.date.issued2022-03
dc.description.abstractContext: Circulating free DNA (cfDNA) analysis has emerged as novel noninvasive diagnostic biomarker in several solid tumors. Raised levels have been reported in several malignancies and may correlate with clinicopathological and treatment response. The current study was designed to assess the diagnostics of cfDNA in different tumor types of malignancies correlating with tumor (T), nodes (N), and metastases (M) stage. Design: Serum samples were collected from treatment naïve cases with histologically diagnosed tumors including 23 brain tumors, 48 breasts, 50 gallbladder carcinoma (GBC), 13 lungs, 68 oral squamous cell carcinoma (OSCC), and 25 normal controls. CfDNA was quantified with real-time polymerase chain reaction (PCR), Invasive ductal carcinoma (IDC) using beta-globin gene amplification. Cut off values for diagnostics were calculated using receiver operating curve analysis. Results: Contrary to other cfDNA studies where it was postulated that cfDNA would not cross the blood–brain barrier and reach the systemic circulation, we found detectable cfDNA in glioma with median (Q1–Q3) of 349.22 ng/ml (19.87–1276.58). Median cfDNA concentration in breast, gallbladder, lung, oral and normal controls was 328.72 (128.38–624.44), 778.50 (589.88–1864.35), 348.73 (194.67–483.61), 386.27 (47.88–959.67), and 74.12 (49.66–120.00), respectively. Grades I and II glioma had significantly lower levels compared to Grades III and IV (P = 0.0001). Significant difference in median cfDNA values in IDC and GBC was observed with increasing tumor grades, stage, T stage, nodal stage and metastasis and with stage of OSCC cases. Conclusion: CfDNA levels showed good diagnostic discrimination in glioma, GBC, breast, lung carcinoma, and OSCC. Significant increase in titers was evident with increase in cancer stage from I to IV in breast, GBC and OSCC.en_US
dc.identifier.affiliationsDepartment of Pathology, Dr. Ram Manohar Lohia Institute of Medical Sciences, Vibhuti Khand, Gomti Nagar, Indiaen_US
dc.identifier.affiliationsDepartment of Neurosurgery, Dr. Ram Manohar Lohia Institute of Medical Sciences, Vibhuti Khand, Gomti Nagar, Indiaen_US
dc.identifier.affiliationsDepartment of Surgical Oncology, Dr. Ram Manohar Lohia Institute of Medical Sciences, Vibhuti Khand, Gomti Nagar, Indiaen_US
dc.identifier.affiliationsDepartment of Radiotherapy, Dr. Ram Manohar Lohia Institute of Medical Sciences, Vibhuti Khand, Gomti Nagar, Indiaen_US
dc.identifier.affiliationsDepartment of Surgery, King George's Medical University, Chowk, Lucknow, Uttar Pradesh, Indiaen_US
dc.identifier.citationKumari S, Mishra S, Husain N, Verma T, Tiwari V, Kaif M, Agarwal A, Rastogi M, Shukla S, Sonkar AA.. Comparison of circulating DNA in malignant neoplasia from diverse locations: Investigating a diagnostic role. Indian Journal of Pathology & Microbiology. 2022 Mar; 65(1): 93-99en_US
dc.identifier.issn0377-4929
dc.identifier.issn0974-5130
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/223176
dc.languageenen_US
dc.publisherWolters Kluwer - Medknowen_US
dc.relation.issuenumber1en_US
dc.relation.volume65en_US
dc.source.urihttps://doi.org/10.4103/IJPM.IJPM_474_20en_US
dc.subjectBiomarkeren_US
dc.subjectcirculating free DNAen_US
dc.subjectDNA quantificationen_US
dc.subjectreal time PCRen_US
dc.titleComparison of circulating DNA in malignant neoplasia from diverse locations: Investigating a diagnostic roleen_US
dc.typeJournal Articleen_US
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