Viral Load-based Evaluation of the Sensitivity of Antigen-based Rapid Detection Assay for SARS-CoV-2

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dc.contributor.authorKhanna, Men_US
dc.contributor.authorBasil, MVen_US
dc.contributor.authorManocha, Nen_US
dc.contributor.authorAgarwal, Nen_US
dc.contributor.authorChauhan, Ven_US
dc.contributor.authorArora, Ren_US
dc.contributor.authorGuleria, Aen_US
dc.contributor.authorKumar, Cen_US
dc.contributor.authorChoudhary, Jen_US
dc.contributor.authorSingh, Aen_US
dc.contributor.authorShrivastava, Ken_US
dc.contributor.authorGoel, Nen_US
dc.contributor.authorSpalgais, Sen_US
dc.contributor.authorMrigpuri, Pen_US
dc.contributor.authorKumar, R.en_US
dc.date.accessioned2023-08-09T06:06:27Z
dc.date.available2023-08-09T06:06:27Z
dc.date.issued2022-09
dc.description.abstractBackground: Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has become a global public health problem. The real-time reverse transcription-polymerase chain reaction (RT-PCR) is the gold standard test for the detection of SARS-CoV-2. However, the assay requires hours to get the final results. Therefore, antigen-based rapid assays are being used extensively to reduce the time. We have evaluated the performance of the antigen-based rapid test for the detection of SARS-CoV-2 virus in comparison with RT-PCR. Materials and methods: Nasopharyngeal and throat swabs were collected from 366 suspected patients of COVID-19 visiting our institute and subjected to qualitative RT-PCR and antigen-based rapid assays to detect the presence of SARS-CoV-2 virus. The sensitivity and specificity of the antigen-based assay were calculated in comparison with RT-PCR. Results: Compared with RT-PCR, sensitivity and specificity of the antigen-based rapid assay were observed to be 70.5% and 98.6%, respectively, in comparison with RT-PCR. However, the sensitivity of antigen-based rapid assay varied significantly with decreasing viral load. The sensitivity of the rapid antigen assay was equivalent to RT-PCR (23/23, 100%) at a higher viral load (Ct value 15�). In contrast, the antigen assay could only detect 3/21 (14.28%) samples with Ct value >30. Conclusion: The antigen-based assay could assist in the rapid screening of a large population. However, the rapid antigen assay might not detect early stages of infection represented by low viral load. Therefore, the antigen-based assay could not replace RT-PCR testing. The study reiterates that all antigen-based negative tests should be confirmed by RT-PCR.en_US
dc.identifier.affiliationsDepartment of Virology Unit, VP Chest Institute, University of Delhi, New Delhi, Indiaen_US
dc.identifier.affiliationsDepartment of Microbiology, VP Chest Institute, University of Delhi, New Delhi, Indiaen_US
dc.identifier.affiliationsDepartment of Pulmonary Medicine, VP Chest Institute, University of Delhi, New Delhi, Indiaen_US
dc.identifier.citationKhanna M, Basil MV, Manocha N, Agarwal N, Chauhan V, Arora R, Guleria A, Kumar C, Choudhary J, Singh A, Shrivastava K, Goel N, Spalgais S, Mrigpuri P, Kumar R.. Viral Load-based Evaluation of the Sensitivity of Antigen-based Rapid Detection Assay for SARS-CoV-2 . Indian Journal of Chest Diseases & Allied Sciences. 2022 Sep; 64(3): 196-198en_US
dc.identifier.issn0377-9343
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/221822
dc.languageenen_US
dc.publisherJaypee Brothers Medical Publishers Pvt. Ltden_US
dc.relation.issuenumber3en_US
dc.relation.volume64en_US
dc.source.urihttps://doi.org/10.5005/jp-journals-11007-0042en_US
dc.subjectAssay sensitivityen_US
dc.subjectCOVID-19 pandemicen_US
dc.subjectRapid antigen testen_US
dc.subjectSARS-CoV-2en_US
dc.subjectViral loaden_US
dc.subjectViral surveillanceen_US
dc.titleViral Load-based Evaluation of the Sensitivity of Antigen-based Rapid Detection Assay for SARS-CoV-2en_US
dc.typeJournal Articleen_US
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