Molecular cloning of human satellite DNA sequences and their use in detecting DNA polymorphism.

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dc.contributor.authorEhtesham, N Zen_US
dc.contributor.authorTalwar, G Pen_US
dc.contributor.authorAli, Aen_US
dc.contributor.authorHasnain, S Een_US
dc.date.accessioned1990-10-01en_US
dc.date.accessioned2009-05-27T09:30:26Z
dc.date.available1990-10-01en_US
dc.date.available2009-05-27T09:30:26Z
dc.date.issued1990-10-01en_US
dc.description.abstractA approximately 400 bp HaeIII human genomic satellite DNA band was cloned into pUC18 to construct a partial library. A fragment of bacteriophage M13 containing a sequence homologous to the human minisatellite core was cloned in pUC18 and was used as a probe to isolate a approximately 350 bp human satellite clone (pTRF5.6) from the partial library. Other clones from this library showed a wide variation in terms of size and hybridization to the pTRF5.6 clone. Human DNA from different individuals was digested with restriction enzymes, Southern transferred and probed with TRF5.6. Individual-specific complex pattern of DNA bands was produced. TRF5.6, therefore, could be useful as a probe for detecting genetic polymorphism.en_US
dc.description.affiliationNational Institute of Immunology, Shaheed Jeet Singh Marg, New Delhi.en_US
dc.identifier.citationEhtesham NZ, Talwar GP, Ali A, Hasnain SE. Molecular cloning of human satellite DNA sequences and their use in detecting DNA polymorphism. Indian Journal of Biochemistry & Biophysics. 1990 Oct; 27(5): 275-9en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/27713
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshDNA, Satellite --geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPolymorphism, Geneticen_US
dc.titleMolecular cloning of human satellite DNA sequences and their use in detecting DNA polymorphism.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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