Type 1–type 2 interferon imbalance in dry eye disease

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dc.contributor.authorPanigrahi, Trailokyanathen_US
dc.contributor.authorD’Souza, Sharonen_US
dc.contributor.authorSuresh Babu, Vishnuen_US
dc.contributor.authorDickman, Mor Men_US
dc.contributor.authorNuijts, Rudy M M Aen_US
dc.contributor.authorSethu, Swaminathanen_US
dc.contributor.authorShetty, Rohiten_US
dc.date.accessioned2023-08-25T06:36:43Z
dc.date.available2023-08-25T06:36:43Z
dc.date.issued2023-04
dc.description.abstractPurpose: Dry eye disease (DED) is characterized by altered ocular surface proinflammatory and antiinflammatory factors. Interferons (IFNs) are a class of pleiotropic cytokines well known for their antimicrobial, inflammatory, and immunomodulatory roles. Hence, this study investigates the ocular surface expression of different types of IFNs in patients with DED. Methods: The cross?sectional, observational study included patients with DED and normal subjects. Conjunctival impression cytology (CIC) samples were obtained from the study subjects (controls, n = 7; DED, n = 8). The mRNA expression levels of type 1 IFN (IFN?, IFN?), type 2 IFN (IFN?), and type 3 IFN (IFN?1, IFN?2, IFN?3) were measured by quantitative PCR (polymerase chain reaction) in CIC samples. IFN? and IFN? expression under hyperosmotic stress was also studied in human corneal epithelial cells (HCECs) in vitro. Results: The mRNA expression levels of IFN? and IFN? were significantly lower and that of IFN? was significantly higher in DED patients compared to healthy controls. The mRNA levels of IFN?, IFN?, and IFN? were significantly lower compared to IFN? in DED patients. An inverse association between tonicity?responsive enhancer?binding protein (TonEBP; hyperosmotic stress maker) and IFN? or IFN? expression and a positive association between TonEBP and IFN? expression was observed in CIC samples. The expression of IFN? was lower than IFN? in HCECs undergoing hyperosmotic stress compared to HCECs without the stress. Conclusion: The presence of an imbalance between type 1 and type 2 IFNs in DED patients suggests newer pathogenic processes in DED, plausible ocular surface infection susceptibility in DED patients, and potential therapeutic targets in the management of DEDen_US
dc.identifier.affiliationsGROW Research Laboratory, Narayana Nethralaya Foundation, Bengaluru, Karnataka, Indiaen_US
dc.identifier.affiliationsDepartment of Cornea and Refractive Surgery, Narayana Nethralaya, Bengaluru, Karnataka, Indiaen_US
dc.identifier.affiliationsDepartment of Ophthalmology, University Eye Clinic Maastricht, Maastricht University Medical Center, Maastricht, Netherlandsen_US
dc.identifier.affiliationsMERLN, Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Maastricht, Netherlandsen_US
dc.identifier.citationPanigrahi Trailokyanath, D’Souza Sharon, Suresh Babu Vishnu, Dickman Mor M, Nuijts Rudy M M A, Sethu Swaminathan, Shetty Rohit. Type 1–type 2 interferon imbalance in dry eye disease. Indian Journal of Ophthalmology. 2023 Apr; 71(4): 1526-1532en_US
dc.identifier.issn1998-3689
dc.identifier.issn0301-4738
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/224961
dc.languageenen_US
dc.publisherAll India Ophthalmological Societyen_US
dc.relation.issuenumber4en_US
dc.relation.volume71en_US
dc.source.urihttps://doi.org/10.4103/IJO.IJO_2842_22en_US
dc.subjectDry eye diseaseen_US
dc.subjecthyperosmotic stressen_US
dc.subjectIFN?en_US
dc.subjectIFN?en_US
dc.subjectIFN?en_US
dc.subjectinterferonsen_US
dc.subjectTonEBPen_US
dc.titleType 1–type 2 interferon imbalance in dry eye diseaseen_US
dc.typeJournal Articleen_US
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