An evaluation of saliva as an alternative to plasma for the detection of hepatitis C virus antibodies.

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dc.contributor.authorMoorthy, Men_US
dc.contributor.authorDaniel, H Den_US
dc.contributor.authorKurian, Gen_US
dc.contributor.authorAbraham, Pen_US
dc.date.accessioned2008-10-01en_US
dc.date.accessioned2009-05-28T09:22:41Z
dc.date.available2008-10-01en_US
dc.date.available2009-05-28T09:22:41Z
dc.date.issued2008-10-01en_US
dc.description.abstractPURPOSE: Seroepidemiological studies on the prevalence of Hepatitis C virus (HCV) in India have been hampered by reluctance of subjects to provide blood samples for testing. We evaluated the use of saliva as an alternate specimen to blood for the detection of antibodies to HCV. METHODS: Chronic liver disease (CLD) patients attending the liver clinic were recruited for this study. A saliva and plasma sample (sample pair) was collected from each patient included in the study. Saliva samples were collected using a commercially available collection device--OmniSal. Sample pairs were tested with an in-use ELISA for the detection of antibodies to HCV (HCV-Ab), with a minor modification in the manufacturer's protocol while testing saliva. The cut-off absorbance value for declaring a sample as positive was determined by receiver operating curve (ROC) analysis. HCV-Ab positivity in saliva was compared with that in plasma as well as with viral load in plasma and infecting genotype of the virus. Sensitivity, specificity, positive and negative predictive values, and correlation coefficients were calculated using Medcalc statistical software. RESULTS: The optimal accuracy indices were: sensitivity-81.6%; specificity-92.5%; PPV-85.1% and NPV-90.5%. No correlation was found between salivary positivity and HCV viral load in plasma or infecting genotype. CONCLUSIONS: The accuracy indices indicate that the assay must be optimized further before it can be recommended for routine use in epidemiological surveys for HCV-Ab.en_US
dc.description.affiliationDepartment of Clinical Virology, Christian Medical College, Vellore, Tamil Nadu, India.en_US
dc.identifier.citationMoorthy M, Daniel HD, Kurian G, Abraham P. An evaluation of saliva as an alternative to plasma for the detection of hepatitis C virus antibodies. Indian Journal of Medical Microbiology. 2008 Oct-Dec; 26(4): 327-32en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/53836
dc.language.isoengen_US
dc.source.urihttps://www.ijmm.orgen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshHepacivirus --classificationen_US
dc.subject.meshHepatitis C --diagnosisen_US
dc.subject.meshHepatitis C Antibodies --analysisen_US
dc.subject.meshHumansen_US
dc.subject.meshPredictive Value of Testsen_US
dc.subject.meshRNA, Viral --blooden_US
dc.subject.meshROC Curveen_US
dc.subject.meshSaliva --immunologyen_US
dc.subject.meshSensitivity and Specificityen_US
dc.titleAn evaluation of saliva as an alternative to plasma for the detection of hepatitis C virus antibodies.en_US
dc.typeEvaluation Studiesen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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