Effect of vaginally administered fumagillin on immunohistochemical distribution of leukemia inhibitory factor, interleukin 6, transforming growth factor beta and vascular endothelial growth factor in implantation stage endometrium of the rhesus monkey.

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dc.contributor.authorKar, Manishaen_US
dc.contributor.authorLalitkumar, P G Len_US
dc.contributor.authorDhawan, Latikaen_US
dc.contributor.authorSengupta, Jayasreeen_US
dc.contributor.authorGhosh, Den_US
dc.date.accessioned2002-01-25en_US
dc.date.accessioned2009-06-01T07:23:48Z
dc.date.available2002-01-25en_US
dc.date.available2009-06-01T07:23:48Z
dc.date.issued2002-01-25en_US
dc.description.abstractIntravaginal administration of an anti-angiogenic agent, fumagillin, during blastocyst implantation inhibits pregnancy establishment in a dose-related manner in the rhesus monkey. In the present study, mated female rhesus monkeys were vaginally inserted with tampons containing vehicle (group 1; n = 5) and test agent (fumagillin, 4 mg/animal; group 2; n = 6) on cycle day 20, and endometrial tissue samples were collected on cycle day 24 from all monkeys and processed for histological examination and immunohistochemical localization for LIF, IL-6, TGF-beta and VEGF. Concentrations of estradiol-17 beta, progesterone and chorionic gonadotrophin in peripheral circulation were determined. From the serum profiles of the hormones, 2 monkeys in group 1, and 1 monkey in group 2 appeared pregnant. However, endometrial morphology revealed histological evidence of pregnancy in 3 out of 6 fumagillin-treated animals. Histometric analysis of immunohistochemical staining in epithelial, stromal and vascular compartments revealed that per cent areas occupied by immunoprecipitate for the cytokines studies did not change in epithelial and stromal compartments, except that for TGF-beta which was higher (P < 0.05) in epithelial compartment in group 2. No change was observed in immunoprecipitation areas for IL-6 in epithelial, stromal and vascular compartments. On the other hand, changes (P < 0.05) for LIF, TGF-beta and VEGF were evident in the vascular compartment. It is possible that disparate responses observed in glandular, stromal and vascular compartments in implantation stage endometrium following fumagillin treatment actually caused from associated decline in progesterone concentration in peripheral circulation. It is also possible that fumagillin, an angiostatic agent, affects the synthesis and secretion of cytokines primarily in the vascular compartment of implantation stage endometrium, and thereby manifests differential responses in epithelial, stromal and vascular compartments.en_US
dc.description.affiliationDepartment of Physiology, All India Institute of Medical Sciences, New Delhi-110 029.en_US
dc.identifier.citationKar M, Lalitkumar PG, Dhawan L, Sengupta J, Ghosh D. Effect of vaginally administered fumagillin on immunohistochemical distribution of leukemia inhibitory factor, interleukin 6, transforming growth factor beta and vascular endothelial growth factor in implantation stage endometrium of the rhesus monkey. Indian Journal of Physiology and Pharmacology. 2002 Jan; 46(1): 25-35en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/108123
dc.language.isoengen_US
dc.source.urihttps://www.ijpp.comen_US
dc.subject.meshAdministration, Intravaginalen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCyclohexanesen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshEmbryo Implantation --drug effectsen_US
dc.subject.meshEndometrium --chemistryen_US
dc.subject.meshEndothelial Growth Factors --analysisen_US
dc.subject.meshFatty Acids, Unsaturated --administration & dosageen_US
dc.subject.meshFemaleen_US
dc.subject.meshGrowth Inhibitors --analysisen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshIntercellular Signaling Peptides and Proteins --analysisen_US
dc.subject.meshInterleukin-6 --analysisen_US
dc.subject.meshLeukemia Inhibitory Factoren_US
dc.subject.meshLymphokines --analysisen_US
dc.subject.meshMacaca mulattaen_US
dc.subject.meshMaleen_US
dc.subject.meshSesquiterpenesen_US
dc.subject.meshTransforming Growth Factor beta --analysisen_US
dc.subject.meshVascular Endothelial Growth Factor Aen_US
dc.subject.meshVascular Endothelial Growth Factorsen_US
dc.titleEffect of vaginally administered fumagillin on immunohistochemical distribution of leukemia inhibitory factor, interleukin 6, transforming growth factor beta and vascular endothelial growth factor in implantation stage endometrium of the rhesus monkey.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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