Measurement of IGF-1, IGFBP-3 and free IGF-1 levels by ELISA in growth hormone (GH) deficient children before and after GH replacement.

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dc.contributor.authorWacharasindhu, Suttipongen_US
dc.contributor.authorAroonparkmongkol, Suphaben_US
dc.contributor.authorSrivuthana, Sumarleeen_US
dc.date.accessioned2009-05-27T17:12:16Z
dc.date.available2009-05-27T17:12:16Z
dc.date.issued2002-09-18en_US
dc.descriptionPublished by the Allergy and Immunology Society of Thailand.en_US
dc.description.abstractSerum insulin-like growth factor-1 (IGF-1) and insulin-like growth factor binding protein-3 (IGFBP-3) levels reflect the growth hormone (GH) status. A few percent of IGF-1 circulate in a free form which is believed to represent the IGF biological activity. We retrospectively studied the changes of serum IGF-1, serum IGFBP-3, and plasma free IGF-1 levels in growth hormone deficient (GHD) children before and after treatment with recombinant human growth hormone (rhGH) for a period of 6 months and 1 year. Twenty-one GHD children (16 boys and 5 girls) who had the mean chronological and bone ages of 7.7 +/- 0.7 and 4.8 +/- 0.6 years, respectively, were treated with a mean rhGH dose of 11.66 +/- 0.42 U/m2 body surface area/week. Serum IGF-1 level increased from 162.5 +/- 42.9 ng/ml before treatment to 252.8 +/- 49.5 ng/ml (p = 0.007) and 282.7 +/- 86.9 ng/ml after treatment for 6 months and 1 year, respectively. Plasma free IGF-1 also increased from 0.38 +/- 0.30 ng/ml before treatment to 1.21 +/- 0.30 (p = 0.001) and 1.17 +/- 0.42 ng/ml after 6 months and 1 year of treatment. However, serum IGFBP-3 did not significantly increase after treatment. In addition, the free/total IGF-1 ratio decreased after treatment with rhGH. The height velocities at 6 months and 1 year after treatment were negatively correlated with plasma free IGF-1 before treatment. In conclusion, therefore, plasma free IGF-1 levels could serve as a good predictor of growth hormone responses. Furthermore, their circulating levels would be modified by serum IGF-1 status, and possibly, IGFBP-3 protease activity.en_US
dc.description.affiliationEndocrine Unit, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.en_US
dc.identifier.citationWacharasindhu S, Aroonparkmongkol S, Srivuthana S. Measurement of IGF-1, IGFBP-3 and free IGF-1 levels by ELISA in growth hormone (GH) deficient children before and after GH replacement. Asian Pacific Journal of Allergy and Immunology. 2002 Sep; 20(3): 155-60en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/36679
dc.language.isoengen_US
dc.subject.meshBiological Markers --blooden_US
dc.subject.meshBody Height --drug effectsen_US
dc.subject.meshChilden_US
dc.subject.meshChild Welfareen_US
dc.subject.meshDwarfism, Pituitary --blooden_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshFemaleen_US
dc.subject.meshGrowth Hormone --therapeutic useen_US
dc.subject.meshHumansen_US
dc.subject.meshInsulin-Like Growth Factor Binding Protein 3 --blooden_US
dc.subject.meshInsulin-Like Growth Factor I --drug effectsen_US
dc.subject.meshMaleen_US
dc.subject.meshRetrospective Studiesen_US
dc.subject.meshStatistics as Topicen_US
dc.subject.meshTime Factorsen_US
dc.subject.meshTreatment Outcomeen_US
dc.titleMeasurement of IGF-1, IGFBP-3 and free IGF-1 levels by ELISA in growth hormone (GH) deficient children before and after GH replacement.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
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