Isolation and in vitro antimalarial activity of hexane extract from Thai Picrasma javanica B1 stembark.

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dc.contributor.authorSaiin, Cen_US
dc.contributor.authorRattanajak, Ren_US
dc.contributor.authorKamchonwongpaisan, Sen_US
dc.contributor.authorIngkaninan, Ken_US
dc.contributor.authorSukontason, Ken_US
dc.contributor.authorBaramee, Aen_US
dc.contributor.authorSirithunyalug, Ben_US
dc.date.accessioned2009-05-27T15:40:48Z
dc.date.available2009-05-27T15:40:48Z
dc.date.issued2003-09-24en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractThe in vitro antimalarial activities against Plasmodium falciparum K1 of four extracts from the stembark of Picrasma javanica B1; ie water, methanol, chloroform and hexane extracts were studied using a modification of the [3H]hypoxanthine incorporation method. It was found that the hexane extract showed in vitro antimalarial activity with IC50 of 3.3 microg/ml. The extract was further fractionated using quick column chromatography, resulting in ten fractions. Fraction V was the most effective against P. falciparum K1 with IC50 of 4.4 microg/ml. Further isolation of fraction V using a column chromatographic technique provided six fractions. According to 1H- and 13C-NMR spectra, it could be concluded that the major compound in fraction V-3 was beta-sitosterol. Unfortunately, the antimalarial activity of beta-sitosterol could not be determined because of its low solubility in DMSO. However, fractions V-2 and V-4 still showed in vitro antimalarial activities with IC50 of 2.8 and 3.4 microg/ml, respectively. The further fractionation of these two active fractions could lead to promising candidates as antimalarial agents.en_US
dc.description.affiliationFaculty of Pharmacy, Chiang Mai University, Chiang Mai, Thailand.en_US
dc.identifier.citationSaiin C, Rattanajak R, Kamchonwongpaisan S, Ingkaninan K, Sukontason K, Baramee A, Sirithunyalug B. Isolation and in vitro antimalarial activity of hexane extract from Thai Picrasma javanica B1 stembark. The Southeast Asian Journal of Tropical Medicine and Public Health. 2003 ; 34 Suppl 2(): 51-5en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/33391
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/publication.htmen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntimalarials --pharmacologyen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshInhibitory Concentration 50en_US
dc.subject.meshMagnetic Resonance Spectroscopyen_US
dc.subject.meshPicrasmaen_US
dc.subject.meshPlant Barken_US
dc.subject.meshPlant Extracts --pharmacologyen_US
dc.subject.meshPlasmodium falciparum --drug effectsen_US
dc.subject.meshThailanden_US
dc.titleIsolation and in vitro antimalarial activity of hexane extract from Thai Picrasma javanica B1 stembark.en_US
dc.typeIn Vitroen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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