Sensitive enzyme-linked immunosorbent assay with urine samples: a tool for surveillance of schistosomiasis japonica.

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dc.contributor.authorItoh, Makotoen_US
dc.contributor.authorOhta, Nobuoen_US
dc.contributor.authorKanazawa, Tamotsuen_US
dc.contributor.authorNakajima, Yasuoen_US
dc.contributor.authorSho, Masanorien_US
dc.contributor.authorMinai, Masaruen_US
dc.contributor.authorDaren, Zhouen_US
dc.contributor.authorChen, Yanen_US
dc.contributor.authorHe, Hongbinen_US
dc.contributor.authorHe, Yong-Kangen_US
dc.contributor.authorZhong, Zhinanen_US
dc.date.accessioned2009-05-27T16:13:25Z
dc.date.available2009-05-27T16:13:25Z
dc.date.issued2003-09-30en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractAn enzyme-linked immunosorbent assay (ELISA) to detect antibodies to Schistosoma japonicum soluble egg antigens (SEA) in un-concentrated urine was developed. The urine ELISA was applied to samples collected in a schistosomiasis-endemic village in China. The levels of anti-SEA antibodies detected in urine correlated well with those obtained with paired serum samples (r = 0.694, p<0.0001). Among 129 serum ELISA positives, 112 (86.8%) were positive by urine ELISA, while all 40 serum ELISA negatives from a non-endemic area were negative. The levels of anti-SEA in urine samples were stable up to 8 weeks of storage at 37 degrees C, with sodium azide as a preservative. Therefore, ELISA with urine samples can be used for the surveillance of schistosomiasis.en_US
dc.description.affiliationDepartment of Parasitology Aichi Medical University School of Medicine, Nagakute, Aichi-ken, Japan.en_US
dc.identifier.citationItoh M, Ohta N, Kanazawa T, Nakajima Y, Sho M, Minai M, Daren Z, Chen Y, He H, He YK, Zhong Z. Sensitive enzyme-linked immunosorbent assay with urine samples: a tool for surveillance of schistosomiasis japonica. The Southeast Asian Journal of Tropical Medicine and Public Health. 2003 Sep; 34(3): 469-72en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/34710
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/2003_34_3/01-3054.pdfen_US
dc.subject.meshAntibodies, Helminth --urineen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assay --methodsen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoglobulin G --urineen_US
dc.subject.meshPopulation Surveillance --methodsen_US
dc.subject.meshSchistosomiasis japonica --diagnosisen_US
dc.subject.meshSensitivity and Specificityen_US
dc.subject.meshSpecimen Handlingen_US
dc.titleSensitive enzyme-linked immunosorbent assay with urine samples: a tool for surveillance of schistosomiasis japonica.en_US
dc.typeEvaluation Studiesen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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