Effect of gallic acid on alkaline phosphatase and peptidase activities in rat intestine.

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dc.contributor.authorMahajan, Nidhi
dc.contributor.authorMahmood, Akhtar
dc.date.accessioned2011-11-11T05:25:20Z
dc.date.available2011-11-11T05:25:20Z
dc.date.issued2009-10
dc.description.abstractGallic acid is a normal constituent of many edible foods, thus directly interacts with epithelial tissue in intestine. In the present study, the effect of gallic acid on intestinal alkaline phosphatase (IAP) and peptidase activities in rat intestine was evaluated. Gallic acid (0.27-0.5 mM) inhibited activities of leucine aminopeptidase (LAP) and -glutamyl transpeptidase (-GTP) by over 90%, compared to controls in rat intestine. In contrast, 0.1-0.6 mM gallic acid either had no effect or stimulated the activity of IAP in rat intestine. The observed inhibition of peptidases by gallic acid was reversible in nature. Kinetic analysis revealed no change in Vmax of LAP (0.42-0.44 units/mg protein) and -GTP (0.22-0.24 units/mg protein), while the values of apparent Km were increased 6-7 fold, exhibiting competitive-type of enzyme inhibition by gallic acid. The values of Ki for LAP and -GTP were 0.037 mM and 0.017 mM, respectively. These observations indicate that gallic acid is a potent inhibitor of brush border peptidases, and thus may interfere in the digestion and absorption of proteins in the intestine.en_US
dc.identifier.citationMahajan Nidhi, Mahmood Akhtar. Effect of gallic acid on alkaline phosphatase and peptidase activities in rat intestine. Indian Journal of Biochemistry & Biophysics. 2009 Oct; 46(5): 378-382.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/135220
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/bitstream/123456789/6295/1/IJBB%2046%285%29%20378-382.pdfen_US
dc.subjectLeucine aminopeptidaseen_US
dc.subject-Glutamyl transpeptidaseen_US
dc.subjectIntestinal alkaline phosphataseen_US
dc.subjectEnzyme inhibitionen_US
dc.subjectGallic aciden_US
dc.subjectRat intestineen_US
dc.subjectBrush border membraneen_US
dc.subject.meshAlkaline Phosphatase --antagonists & inhibitors
dc.subject.meshAlkaline Phosphatase --metabolism
dc.subject.meshAnimals
dc.subject.meshDose-Response Relationship, Drug
dc.subject.meshEnzyme Inhibitors --pharmacology
dc.subject.meshGallic Acid --pharmacology
dc.subject.meshIntestines --drug effects
dc.subject.meshIntestines --enzymology
dc.subject.meshIntestines --metabolism
dc.subject.meshKinetics
dc.subject.meshLeucyl Aminopeptidase --antagonists & inhibitors
dc.subject.meshLeucyl Aminopeptidase --metabolism
dc.subject.meshMale
dc.subject.meshRats
dc.subject.meshRats, Wistar
dc.subject.meshgamma-Glutamyltransferase --antagonists & inhibitors
dc.subject.meshgamma-Glutamyltransferase --metabolism
dc.titleEffect of gallic acid on alkaline phosphatase and peptidase activities in rat intestine.en_US
dc.typeArticleen_US
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