In vitro cytotoxic activity of leaves extracts of Holarrhena antidysenterica against some human cancer cell lines.

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dc.contributor.authorSharma, Vikas
dc.contributor.authorHussain, Shabir
dc.contributor.authorBakshi, Manish
dc.contributor.authorBhat, Neha
dc.contributor.authorSaxena, Ajit Kumar
dc.date.accessioned2014-12-26T07:55:03Z
dc.date.available2014-12-26T07:55:03Z
dc.date.issued2014-02
dc.description.abstractIn vitro cytotoxic potential of extracts (95% and 50% ethanolic extract and hot water extract at concentration of 100 µg/ml) from leaves of Holarrhena antidysenterica was evaluated against fourteen human cancer cell lines — A-549, COLO-205, DU-145, HeLa, HEP-2, IMR-32, KB, MCF-7, NCI-H23, OVCAR-5, SiHa, SK-N-MC, SW-620 and ZR-75-1 from nine different tissues (breast, colon, cervix, CNS, lung, liver, oral, ovary and prostate) using SRB assay. The 95% ethanolic extract displayed maximum anti-proliferative effect in the range of 73-92% against eight human cancer cell lines, while 50% ethanolic extract showed cytotoxic activity in the range of 70-94% against seven human cancer cell lines. However, the hot water extract did not show any activity. Among the fractions of 95% and 50% ethanolic extract, significant cytotoxic activity was found in the chloroform soluble fraction of 95% ethanolic extract at 100 µg/ml; it inhibited the growth in the range of 71-99% of seven human cancer cell lines from five different tissues viz., OVCAR-5 (ovary), HT-29 (colon), SK-N-MC (neuroblastoma), HEP-2 (liver), COLO-205 (colon), NIH-OVCAR-3 (ovary) and A-549 (lung). The cytotoxic activity of chloroform soluble fraction was found to be higher than 5-flurouracil, adriamycin, mitomycin-c and paclitaxel (anticancer drugs used as positive controls). Further in vivo studies and identification of active components from the chloroform fraction and their exact mechanism of action could be useful in designing new anticancer therapeutic agents.en_US
dc.identifier.citationSharma Vikas, Hussain Shabir, Bakshi Manish, Bhat Neha, Saxena Ajit Kumar. In vitro cytotoxic activity of leaves extracts of Holarrhena antidysenterica against some human cancer cell lines. Indian Journal of Biochemistry & Biophysics. 2014 Feb; 51(1): 46-51.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/154230
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/27287en_US
dc.subjectHolarrhena antidysentericaen_US
dc.subjectCytotoxic activityen_US
dc.subjectHuman cancer cellsen_US
dc.subjectSRB assayen_US
dc.subject.meshAntineoplastic Agents --isolation & purification
dc.subject.meshAntineoplastic Agents --pharmacology
dc.subject.meshCell Line, Tumor
dc.subject.meshCell Proliferation --drug effects
dc.subject.meshHolarrhena --chemistry
dc.subject.meshHumans
dc.subject.meshPlant Extracts --isolation & purification
dc.subject.meshPlant Extracts --pharmacology
dc.subject.meshPlant Leaves --chemistry
dc.titleIn vitro cytotoxic activity of leaves extracts of Holarrhena antidysenterica against some human cancer cell lines.en_US
dc.typeArticleen_US
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