Modulation of alpha1beta1 integrin mediated adhesion of hepatocytes to collagen IV and laminin by divalent cations.

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dc.contributor.authorMenon, Ben_US
dc.contributor.authorKumar, N Aen_US
dc.contributor.authorSudhakaran, P Ren_US
dc.date.accessioned2001-06-06en_US
dc.date.accessioned2009-05-27T09:26:48Z
dc.date.available2001-06-06en_US
dc.date.available2009-05-27T09:26:48Z
dc.date.issued2001-06-06en_US
dc.description.abstractCell matrix interactions play a critical role in hepatic development and regeneration after acute injury. These interactions are mediated by transmembrane receptors belonging mainly to the integrin family. We have tried to assess the role of divalent cations in mediating attachment of hepatocytes to matrix proteins like collagen IV (Col IV) and laminin (Ln). The three cations examined viz. Ca2+, Mg2+ and Mn2+ showed attachment promoting activity. Since alpha1beta1 integrin is a common receptor for col IV and LN in liver, the effect of cations in its binding to these matrix proteins was studied. Although cations in general enhanced the binding, different cations exhibited differential effect in promoting the binding for different ligands. Mg2+ ions were more effective in promoting the binding of alpha1beta1 integrin to col IV but Ca2+ proved to be more effective one for Ln. Kinetic analysis of binding in dot blot assays using different concentrations of cations showed that while Mg2+ was active at low concentrations Ca2+ and Mn2+ promoted the binding more at higher concentrations. Absence of competitive effect in binding studies showed that they bind at different sites on the receptor. Differential effects of cations in promoting the binding of alpha1beta1 integrin to Col IV and Ln suggest that changes in level of diffusible cations can modulate affinity of the common receptor alpha1beta1 integrin to its ligands and can influence adhesion of hepatic cells to different matrix proteins during hepatic development and regeneration.en_US
dc.description.affiliationDepartment of Biochemistry, University of Kerala, Kariavattom, Trivandrum, India.en_US
dc.identifier.citationMenon B, Kumar NA, Sudhakaran PR. Modulation of alpha1beta1 integrin mediated adhesion of hepatocytes to collagen IV and laminin by divalent cations. Indian Journal of Biochemistry & Biophysics. 2001 Jun; 38(3): 159-65en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/27532
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBinding, Competitiveen_US
dc.subject.meshCationsen_US
dc.subject.meshCell Adhesionen_US
dc.subject.meshCollagen Type IV --chemistryen_US
dc.subject.meshDimerizationen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshHepatocytes --metabolismen_US
dc.subject.meshIntegrin alpha1beta1en_US
dc.subject.meshIntegrins --chemistryen_US
dc.subject.meshKineticsen_US
dc.subject.meshLaminin --chemistryen_US
dc.subject.meshLiver --metabolismen_US
dc.subject.meshMagnesium --chemistryen_US
dc.subject.meshManganese --chemistryen_US
dc.subject.meshProtein Bindingen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.titleModulation of alpha1beta1 integrin mediated adhesion of hepatocytes to collagen IV and laminin by divalent cations.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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