OXA beta-lactamase-mediated carbapenem resistance in Acinetobacter baumannii.

Simple item page
dc.contributor.authorAmudhan, S M
dc.contributor.authorSekar, U
dc.contributor.authorArunagiri, K
dc.contributor.authorSekar, B
dc.date.accessioned2012-12-13T08:30:26Z
dc.date.available2012-12-13T08:30:26Z
dc.date.issued2011-07
dc.description.abstractObjectives: Acinetobacter baumannii is a significant pathogen in health care settings. In recent years, an increase in carbapenem resistance among A. baumannii due to Ambler class B metallo-beta-lactamases or class D OXA carbapenamases has been reported. In this study we detected the presence of OXA carbapenamases and coproduction of metallo-beta-lactamases (blaVIM and blaIMP ) by phenotypic and genotypic methods in carbapenem resistant clinical isolates of Acinetobacter baumannii. Materials and Methods: A total of 116 consecutive, non-duplicate carbapenem resistant A. baumannii isolated from various clinical specimens were included in the study. The modified Hodge test and inhibitor potentiated disk diffusion tests were done for the screening of carbapenamase and metallo-beta-lactamase production, respectively. Polymerase chain reaction (PCR) was performed for the detection of OXA (blaOXA 23 like, blaOXA 24 like, blaOXA-51 like and blaOXA-58 like genes) and metallo-beta-lactamases (blaVIM and blaIMP ) genes. Gene sequencing was performed for representative isolates. Results: Among 116 A. baumannii, OXA genes were detected in 106 isolates. BlaOXA 51 like (n = 99) and blaOXA -23 like (n = 95) were the most common and they coexisted in 89 isolates. blaOXA-24 like gene was detected in two isolates of which one also carried blaOXA-51 like and blaOXA-58 like genes. The modified Hodge test was positive in 113 isolates. The metallo-beta-lactamase screening test was positive in 92 isolates. blavim was detected in 54 isolates of which 1 also carried the blaIMP gene. Conclusions: blaOXA-23 like and bla OXA 51 like genes are the most common types of OXA carbapenamases while the blaVIM type is the most common type of metallo-beta-lactamase contributing to carbapenem resistance in clinical isolates of A. baumannii. The coproduction of OXA and metallo-beta-lactamases is not an uncommon phenomenon in A. baumannii.en_US
dc.identifier.citationAmudhan S M, Sekar U, Arunagiri K, Sekar B. OXA beta-lactamase-mediated carbapenem resistance in Acinetobacter baumannii. Indian Journal of Medical Microbiology. 2011 Jul-Sept; 29(3): 269-274.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/143829
dc.language.isoenen_US
dc.source.urihttps://www.ijmm.org/article.asp?issn=0255-0857;year=2011;volume=29;issue=3;spage=269;epage=274;aulast=Amudhanen_US
dc.subjectAcinetobacter baumanniien_US
dc.subjectbla OXAen_US
dc.subjectcarbapenamasesen_US
dc.subjectmodified Hodge testen_US
dc.subjectminimum inhibitory concentrationen_US
dc.subject.meshAcinetobacter Infections --microbiology
dc.subject.meshAcinetobacter baumannii --drug effects
dc.subject.meshAcinetobacter baumannii --enzymology
dc.subject.meshAcinetobacter baumannii --isolation & purification
dc.subject.meshAnti-Bacterial Agents --pharmacology
dc.subject.meshCarbapenems --pharmacology
dc.subject.meshHumans
dc.subject.meshMicrobial Sensitivity Tests
dc.subject.meshPolymerase Chain Reaction
dc.subject.meshSequence Analysis, DNA
dc.subject.meshbeta-Lactam Resistance
dc.subject.meshbeta-Lactamases --genetics
dc.subject.meshbeta-Lactamases --metabolism
dc.titleOXA beta-lactamase-mediated carbapenem resistance in Acinetobacter baumannii.en_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ijmm2011v29n3p269.pdf
Size:
811.16 KB
Format:
Adobe Portable Document Format
Description:
Journal article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Indian Journal of Medical Microbiology