Improvement of growth of Plasmodium falciparum fresh clinical isolates by using an established serum-free medium, GIT.

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dc.contributor.authorFuruta, Ten_US
dc.contributor.authorKimura, Men_US
dc.contributor.authorTsunoda, Ten_US
dc.contributor.authorKikuchi, Ten_US
dc.contributor.authorKojima, Sen_US
dc.date.accessioned2009-05-27T15:10:18Z
dc.date.available2009-05-27T15:10:18Z
dc.date.issued2000-09-06en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractIn the present study, we have tried to establish continuous cultures of fresh clinical isolates of P. falciparum by using a serum-free medium, GIT. To examine the ability of GIT to support the parasite growth, the growth of various P. falciparum isolates including two laboratory strains of P. falciparum, FCR3 and K1 was compared in both of GIT and RPMI 1640 medium supplemented by 10% human serum (RPMI-HS). Growth rates of various P. falciparum expressed as fold increases were compared in GIT and RPMI-HS, and the maximum growth rates of P. falciparum were 72 in GIT and 35 in RPMI-HS during the culture for 8 days. Growth rate of the clinical isolates varied individually in both culture media, with average growth rates of parasites being 15.9 in GIT and 8.8 in RPMI-HS, respectively (not significant). Growth rates of FCR3 and K1 strains were 28.0 and 6.6 in GIT, and 10 and 7.5 in RPMI-HS. After 30 days culture of P. falciparum in GIT, 9 of 12 clinical isolates still continuously propagated but other three isolates disappeared. Despite variation of the P. falciparum isolates in their abilities to multiply in GIT, our experiments suggested that GIT is useful for culture of fresh clinical isolates of P. falciparum that are derived from geographically distinct areas as well as laboratory strains used commonly in laboratory research.en_US
dc.description.affiliationDepartment of Parasitology, The Institute of Medical Science, The University of Tokyo, Japan.en_US
dc.identifier.citationFuruta T, Kimura M, Tsunoda T, Kikuchi T, Kojima S. Improvement of growth of Plasmodium falciparum fresh clinical isolates by using an established serum-free medium, GIT. The Southeast Asian Journal of Tropical Medicine and Public Health. 2000 Sep; 31(3): 606-8en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/32099
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/2000_31_3/37-2462.pdfen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCulture Media, Serum-Freeen_US
dc.subject.meshErythrocytes --parasitologyen_US
dc.subject.meshHumansen_US
dc.subject.meshMalaria, Falciparum --parasitologyen_US
dc.subject.meshPlasmodium falciparum --growth & developmenten_US
dc.titleImprovement of growth of Plasmodium falciparum fresh clinical isolates by using an established serum-free medium, GIT.en_US
dc.typeIn Vitroen_US
dc.typeJournal Articleen_US
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