The preservation method and timing on accuracy of manual leukocytes counts in synovial fluid.

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dc.contributor.authorKoolvisoot, Ajcharaen_US
dc.contributor.authorRungbanaphan, Umpaiwanen_US
dc.contributor.authorKatchamat, Wanrachadaen_US
dc.contributor.authorChinsawangwatanakul, Wimolen_US
dc.date.accessioned2009-05-27T20:14:25Z
dc.date.available2009-05-27T20:14:25Z
dc.date.issued2006-11-28en_US
dc.descriptionChotmaihet Thangphaet.en_US
dc.description.abstractOBJECTIVES: To compare the efficacy of heparin and EDTA and determine the impact of time delays in stabilizing leukocyte counts in synovial fluid. MATERIAL AND METHOD: 33 specimens were collected in heparin-preserved and EDTA-preserved containers. Total cell count was performed manually at 1 hour and 24 hours. Correlation between cell counts from both preservatives and the leukocyte number at 1 hour and 24 hours were analyzed by means of agreement measurement. RESULTS: There were good correlations between the leukocyte numbers from the specimens preserved by heparin and EDTA (ICC = 0.889, r = 0.879, P < 0.0001 at 1 hour and ICC = 0.822, r = 0.693, p < 0.0001 at 24 hour). At 24 hours, total cell counts from EDTA-preserved samples were comparable to those obtained at 1 hour (ICC = 0.985, r = 0. 986, p < 0. 0001) and were not different from those of the heparinized samples (ICC = 0.833, r = 0. 751, p < 0.0001) but the ICC value was higher. CONCLUSION: EDTA was as effective as heparin for preservation of synovial fluid. Therefore, it can be used routinely as a preservative of synovial fluid.en_US
dc.description.affiliationDepartment of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand. srakv@mahidol.ac.then_US
dc.identifier.citationKoolvisoot A, Rungbanaphan U, Katchamat W, Chinsawangwatanakul W. The preservation method and timing on accuracy of manual leukocytes counts in synovial fluid. Journal of the Medical Association of Thailand. 2006 Nov; 89 Suppl 5(): S187-94en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/42629
dc.language.isoengen_US
dc.source.urihttps://www.mat.or.th/journal/all.phpen_US
dc.subject.meshCross-Sectional Studiesen_US
dc.subject.meshEdetic Aciden_US
dc.subject.meshFemaleen_US
dc.subject.meshHeparinen_US
dc.subject.meshHumansen_US
dc.subject.meshLeukocytes --chemistryen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshPreservation, Biologicalen_US
dc.subject.meshSynovial Fluid --chemistryen_US
dc.subject.meshTime Factorsen_US
dc.titleThe preservation method and timing on accuracy of manual leukocytes counts in synovial fluid.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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