Development and evaluation of co-agglutination test to detect rotavirus antigens in stools of patients with diarrhoea.

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dc.contributor.authorIslam, M Nen_US
dc.contributor.authorHossain, M Aen_US
dc.contributor.authorRahman, Men_US
dc.contributor.authorYasmin, Men_US
dc.contributor.authorAlam, A Nen_US
dc.contributor.authorHoque, Men_US
dc.contributor.authorSattar, Hen_US
dc.date.accessioned1995-04-01en_US
dc.date.accessioned2009-05-27T03:47:20Z
dc.date.available1995-04-01en_US
dc.date.available2009-05-27T03:47:20Z
dc.date.issued1995-04-01en_US
dc.descriptionBangladesh Medical Research Council Bulletin.en_US
dc.description.abstractRotavirus is the most common cause of acute diarrhoea in infants and children in both the developed and developing countries including Bangladesh. Information about rotavirus diarrhoea in Bangladesh is insufficient primarily due to the lack of diagnostic facilities due to the high cost of reagents and equipment and lack of skilled personnel. A simple, suitable and less costly technique of co-agglutination test using protein-A secreting staphylococci was developed and evaluated against a commercially available ELISA kit to detect rotavirus antigen in stools of patients with diarrhoea. Staphylococcus cowan strain 1 was grown and coated with rabbit antisera raised against RV5 and SA11 rotavirus strains. The antibody-coated staphylococci were agglutinated specifically by rotavirus present in faecal samples within one or two minutes. A total of 1332 stool specimens were tested by co-agglutination and ELISA, of which 210 (15.77%) were positive by ELISA and 276 (20.72%) by co-agglutination test. Compared to ELISA, sensitivity of co-agglutination test was 76.19%, specificity 89.66% and predictive values of a positive and a negative test were 57.97% and 95.26% respectively. The results indicate that the co-agglutination test is a simple and suitable technique for rapid screening of rotavirus infection which could be adopted in clinical practice.en_US
dc.description.affiliationDepartment of Virology, Institute of Postgraduate Medicine and Research, Dhaka Bangladesh.en_US
dc.identifier.citationIslam MN, Hossain MA, Rahman M, Yasmin M, Alam AN, Hoque M, Sattar H. Development and evaluation of co-agglutination test to detect rotavirus antigens in stools of patients with diarrhoea. Bangladesh Medical Research Council Bulletin. 1995 Apr; 21(1): 11-7en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/434
dc.language.isoengen_US
dc.source.urihttps://www.banglajol.info/index.php/BMRCB/indexen_US
dc.subject.meshAgglutination Testsen_US
dc.subject.meshAntigens, Viral --analysisen_US
dc.subject.meshDiarrhea --virologyen_US
dc.subject.meshFeces --virologyen_US
dc.subject.meshHumansen_US
dc.subject.meshRotavirus --immunologyen_US
dc.titleDevelopment and evaluation of co-agglutination test to detect rotavirus antigens in stools of patients with diarrhoea.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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