Improvised microinjection technique for mosquito vectors.
| dc.contributor.author | Kumar, S Sampath | |
| dc.contributor.author | Puttaraju, H P | |
| dc.date.accessioned | 2013-08-02T04:39:19Z | |
| dc.date.available | 2013-08-02T04:39:19Z | |
| dc.date.issued | 2012-12 | |
| dc.description.abstract | Background & objectives: Bio-manipulation technique is of primary importance during the development of transgenic mosquitoes. The study describes the variable factors that influence the viability of medically important mosquito vectors during microinjection. Methods: Three mosquito vectors belonging to the genus Aedes, Anopheles and Culex were microinjected at different developmental stages of their life cycle viz., egg, larvae, pupae and adult. Results: The improvisations revealed an increased survivability of biomanipulated mosquitoes during the embryonic and adult microinjection. The study of injecting larvae and pupae resulted in poor survivability. Interpretation & conclusions: The microinjection protocol was successfully tested on three important mosquito vectors. The critical period after biomanipulation which contributes heavily for the survivability factor was evaluated. The results provide a common protocol for biomanipulation of three mosquito vectors with enhanced survivability. | en_US |
| dc.identifier.citation | Kumar S Sampath, Puttaraju H P. Improvised microinjection technique for mosquito vectors. Indian Journal of Medical Research. 2012 Dec; 136(6): 971-978. | en_US |
| dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/147660 | |
| dc.language.iso | en | en_US |
| dc.source.uri | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3612326/?report=classic | en_US |
| dc.subject | Bio-manipulation | en_US |
| dc.subject | critical period | en_US |
| dc.subject | microinjection | en_US |
| dc.subject | mosquito vectors | en_US |
| dc.subject | survivability | en_US |
| dc.title | Improvised microinjection technique for mosquito vectors. | en_US |
| dc.type | Article | en_US |