Spinal cord injury-induced astrocyte migration and glial scar formation: Effects of magnetic stimulation frequency.

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dc.contributor.authorLi, Zhe
dc.contributor.authorFang, Zheng-Yu
dc.contributor.authorXiong, Liang
dc.contributor.authorHuang, Xiao-Lin
dc.date.accessioned2011-11-15T05:03:02Z
dc.date.available2011-11-15T05:03:02Z
dc.date.issued2010-12
dc.description.abstractThe effects of magnetic stimulation on spinal cord injury-induced migration of white matter astrocytes were studied using an established animal model. Ethidium bromide was injected into the dorsal spinal cord funiculus of adult Sprague-Dawley rats on the left side at T10-11. Animals then received 1.52 Tesla-pulsed magnetic stimulation for 5 min at different frequencies (0-20 Hz) for 14 consecutive days. Selected animals received the non-competitive MEK1/2 inhibitor U0126 (10 μM), prior to stimulation at 10 Hz. Lesion volumes were measured in hematoxylin/eosin-stained sections. Expression of glial fibrillary acidic protein (GFAP), microtubule associated protein-2 (MAP-2) and extra-cellular signal-regulated kinase1/2 (ERK1/2) near the epicenter of injury was examined by Western blotting with quantification using an image analysis system. Lesion volumes decreased and GFAP and p-ERK1/2 expression increased with increasing magnetic stimulation frequency (0-10 Hz). MAP-2 expression was not affected at any frequency. Pretreatment with U0126 reduced GFAP and ERK1/2 expression and increased lesion volumes in response to stimulation at 10 Hz. It is concluded that magnetic stimulation increases the migration of astrocytes to spinal cord lesions. Activation of the ERK1/2 signaling pathway is proposed to mediate astrocyte migration and glial scar formation in response to spinal cord injury.en_US
dc.identifier.citationLi Zhe, Fang Zheng-Yu, Xiong Liang, Huang Xiao-Lin. Spinal cord injury-induced astrocyte migration and glial scar formation: Effects of magnetic stimulation frequency. Indian Journal of Biochemistry & Biophysics. 2010 Dec; 47(6): 359-363.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/135288
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/bitstream/123456789/10859/1/IJBB%2047%286%29%20359-363.pdfen_US
dc.subjectAstrocyte migrationen_US
dc.subjectExtracellular signal-regulated kinase1/2 pathwayen_US
dc.subjectMagnetic stimulationen_US
dc.subjectSpinal cord injuryen_US
dc.subjectGlial scar formationen_US
dc.subject.meshAnimals
dc.subject.meshAstrocytes --pathology
dc.subject.meshCell Movement
dc.subject.meshCicatrix --pathology
dc.subject.meshDisease Models, Animal
dc.subject.meshGlial Fibrillary Acidic Protein --metabolism
dc.subject.meshMAP Kinase Signaling System
dc.subject.meshMagnetic Field Therapy --methods
dc.subject.meshMale
dc.subject.meshMicrotubule-Associated Proteins --metabolism
dc.subject.meshNeuroglia --pathology
dc.subject.meshRats
dc.subject.meshRats, Sprague-Dawley
dc.subject.meshSpinal Cord Injuries --metabolism
dc.subject.meshSpinal Cord Injuries --pathology
dc.subject.meshSpinal Cord Injuries --therapy
dc.titleSpinal cord injury-induced astrocyte migration and glial scar formation: Effects of magnetic stimulation frequency.en_US
dc.typeArticleen_US
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