IgM capture ELISA for detection of IgM antibodies to dengue virus: comparison of 2 formats using hemagglutinins and cell culture derived antigens.

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dc.contributor.authorCardosa, M Jen_US
dc.contributor.authorTio, P Hen_US
dc.contributor.authorNimmannitya, Sen_US
dc.contributor.authorNisalak, Aen_US
dc.contributor.authorInnis, Ben_US
dc.date.accessioned2009-05-27T15:04:53Z
dc.date.available2009-05-27T15:04:53Z
dc.date.issued1992-12-01en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractThe highly sensitive AFRIMS format IgM capture ELISA for the diagnosis of dengue virus infections requires the use of mouse brain derived hemagglutinins and consequently also the use of 20% acetone extracted normal human serum to eliminate high background. These reagents are not always easily available and we have thus compared the AFRIMS format with another published format which uses cell culture derived antigens (culture fluid, CF, format) in order to determine if it is reasonable to use cell culture derived antigens in situations where hemagglutinins and normal human serum are difficult to obtain. The study shows that using AFRIMS results as the reference point, the CF format described here has a sensitivity of 90% and a specificity of 96%.en_US
dc.description.affiliationSchool of Pharmaceutical Sciences, Universiti Sains Malaysia, Penang.en_US
dc.identifier.citationCardosa MJ, Tio PH, Nimmannitya S, Nisalak A, Innis B. IgM capture ELISA for detection of IgM antibodies to dengue virus: comparison of 2 formats using hemagglutinins and cell culture derived antigens. The Southeast Asian Journal of Tropical Medicine and Public Health. 1992 Dec; 23(4): 726-9en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/31863
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/publication.htmen_US
dc.subject.meshAntibodies, Viral --blooden_US
dc.subject.meshAntigens, Viral --diagnostic useen_US
dc.subject.meshDengue --diagnosisen_US
dc.subject.meshDengue Virus --immunologyen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assay --methodsen_US
dc.subject.meshEvaluation Studies as Topicen_US
dc.subject.meshHemagglutination Inhibition Testsen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoglobulin M --blooden_US
dc.subject.meshSensitivity and Specificityen_US
dc.titleIgM capture ELISA for detection of IgM antibodies to dengue virus: comparison of 2 formats using hemagglutinins and cell culture derived antigens.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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