Larvicidal activity of Artemisia annua L. callus culture against Anopheles stephensi larvae.
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Date
2009-05
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Abstract
The emergence of resistance by both Plasmodium falciparum and Anopheles stephensi made the search for an alternative
environmentally safe plant based insecticide inevitable. Artemisia annua is a well known antimalarial. Present study is an attempt to induce
callus production from young leaves of Artemisia annua plant and study its larvicidal activity against larvae of Anopheles stephensi. Callus was
initiated by using different concentrations of auxins and cytokinins. A suitable culture media was standardized for optimal growth of callus.
Healthy callus cultures were obtained in the slightly modified Murashige and Skoog’s medium + NAA and BAP (0.03 and 0.2 mg l-1 respectively)
+ Sucrose 20 gm l-1 + Agar 8 gm l-1 within 28 days of inoculation. Callus was successively extracted in order of increasing polarity of solvents.
Larvicidal activity, in terms of lethal concentration (LC50) of the callus extract in chloroform was calculated to be 18.45 ± 0.75 ppm after 72 hr
against third instar larvae of A. stephensi.
Description
Keywords
Artemisia annua, Callus cultures, Larvicidal component
Citation
Bartarya Roli, Srivastava Anamika, Tonk Shobhita, Bhatnagar V P, Srivastava S S, Kumari K Maharaj. Larvicidal activity of Artemisia annua L. callus culture against Anopheles stephensi larvae. Journal of Environmental Biology. 2009 May; 30(3): 395-398.