Adaptation of a chemiluminescent DNA detection system to strongly positively charged nylon membranes: single copy gene detection in human genome.

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dc.contributor.authorChattopadhyay, Pen_US
dc.contributor.authorSinha, Sen_US
dc.date.accessioned1995-06-01en_US
dc.date.accessioned2009-05-27T09:03:45Z
dc.date.available1995-06-01en_US
dc.date.available2009-05-27T09:03:45Z
dc.date.issued1995-06-01en_US
dc.description.abstractNonradioactive methods for detection of genes by Southern hybridization are limited to neutral or positively charged membranes which are supplied or explicitly recommended by the manufacturers of the chemiluminescent detection systems. The results of such kits with a known strongly positively charged nylon membrane (Genescreen plus) are disappointing. The adaption of a chemiluminescent detection system to such a strongly positively charged nylon membrane is demonstrated in this communication. The modifications include a blocking step (1% SDS) before prehybridization, use of 5% SDS during hybridization, and 5% SDS and 1% BSA during detection. The increased sensitivity of such a highly positively charged nylon membrane enabled us to detect 2.5 pg of target DNA. The detection of both the alleles of a single copy polymorphic target (YNZ22) in the human genome is also shown.en_US
dc.description.affiliationDepartment of Biochemistry, All India Institute of Medical Sciences, New Delhi.en_US
dc.identifier.citationChattopadhyay P, Sinha S. Adaptation of a chemiluminescent DNA detection system to strongly positively charged nylon membranes: single copy gene detection in human genome. Indian Journal of Biochemistry & Biophysics. 1995 Jun; 32(3): 152-5en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/26325
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshCationsen_US
dc.subject.meshChemiluminescent Measurementsen_US
dc.subject.meshDNA --analysisen_US
dc.subject.meshGene Dosageen_US
dc.subject.meshGenome, Humanen_US
dc.subject.meshHumansen_US
dc.subject.meshMembranes, Artificialen_US
dc.subject.meshNylonsen_US
dc.subject.meshSensitivity and Specificityen_US
dc.titleAdaptation of a chemiluminescent DNA detection system to strongly positively charged nylon membranes: single copy gene detection in human genome.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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