Characterization of Streptococcus pneumoniae ophthalmic, systemic & commensal isolates by pulsed-field gel electrophoresis & ribotyping.

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dc.contributor.authorKar, Upendra Ken_US
dc.contributor.authorSatpathy, Gitaen_US
dc.contributor.authorDas, B Ken_US
dc.contributor.authorPanda, S Ken_US
dc.date.accessioned2008-02-12en_US
dc.date.accessioned2009-05-27T06:32:13Z
dc.date.available2008-02-12en_US
dc.date.available2009-05-27T06:32:13Z
dc.date.issued2008-02-12en_US
dc.description.abstractBACKGROUND & OBJECTIVE: Streptococcus pneumoniae is common in ocular and systemic infections and is a part of normal nasopharyngeal flora. Very few studies regarding genetic analysis of S. pneumoniae isolates causing eye infections are available. This study was undertaken to do pulse field gel electrophoresis (PFGE) analysis and ribotyping of S. pneumoniae isolates obtained from eye infections, systemic infections and nasopharyngeal flora. METHODS: Sixty one well characterized S. pneumoniae isolates (38 from ophthalmic infections, 9 from systemic infections and 14 commensals) were characterized using PFGE of the whole genome after SmaI, restriction enzyme digestion and conventional ribotyping using Escherichia coli rRNA operon as the probe. Phylogenetic tree was drawn using unweighted pair group method analysis (UPGMA). RESULTS: The 38 S. pneumoniae isolates from eye infections belonging to 15 serotypes were placed in to 11 PFGE types and 15 ribotypes. The 9 systemic isolates (7 seotypes) were distributed in 7 PFGE types and 6 ribotypes. The 14 commensal isolates were placed in 11 serotypes, 5 PFGE types and 6 ribotypes. Most of the PFGE types and ribotypes consisting of ocular isolates also contained systemic and commensal isolates. INTERPRETATION & CONCLUSION: Considerable genetic similarity was observed between the isolates from ocular and systemic infections and those colonized in nasopharynx. PFGE analysis could differentiate majority of the isolates according to site of infections. There was a considerable DNA polymorphism within the studied bacterial population.en_US
dc.description.affiliationDepartment of Ocular Microbiology, Dr RP Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, India.en_US
dc.identifier.citationKar UK, Satpathy G, Das BK, Panda SK. Characterization of Streptococcus pneumoniae ophthalmic, systemic & commensal isolates by pulsed-field gel electrophoresis & ribotyping. Indian Journal of Medical Research. 2008 Feb; 127(2): 171-7en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/16721
dc.language.isoengen_US
dc.source.urihttps://icmr.nic.in/ijmr/ijmr.htmen_US
dc.subject.meshBacterial Typing Techniquesen_US
dc.subject.meshDNA --metabolismen_US
dc.subject.meshDNA, Bacterial --metabolismen_US
dc.subject.meshElectrophoresis, Gel, Pulsed-Fielden_US
dc.subject.meshEye Infections --microbiologyen_US
dc.subject.meshGenes, Bacterialen_US
dc.subject.meshHumansen_US
dc.subject.meshModels, Geneticen_US
dc.subject.meshMolecular Weighten_US
dc.subject.meshPhylogenyen_US
dc.subject.meshPneumococcal Infections --microbiologyen_US
dc.subject.meshPolymorphism, Geneticen_US
dc.subject.meshRibotyping --methodsen_US
dc.subject.meshSoftwareen_US
dc.subject.meshStreptococcus pneumoniae --metabolismen_US
dc.titleCharacterization of Streptococcus pneumoniae ophthalmic, systemic & commensal isolates by pulsed-field gel electrophoresis & ribotyping.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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