Preliminary study on somatic cell nuclear transfer in rabbits in Thailand.

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dc.contributor.authorTechakumphu, Mongkolen_US
dc.contributor.authorNumchaisrika, Praneeen_US
dc.contributor.authorSuwajanakorn, Somchaien_US
dc.contributor.authorPruksananonda, Kamtornen_US
dc.contributor.authorBoonkasemsanti, Wisuten_US
dc.contributor.authorAhnonkitpanit, Vichudaen_US
dc.contributor.authorVirutamasen, Pramuanen_US
dc.date.accessioned2009-05-27T18:01:37Z
dc.date.available2009-05-27T18:01:37Z
dc.date.issued2003-03-22en_US
dc.descriptionChotmaihet Thangphaet.en_US
dc.description.abstractThe objective of the study was to develop the somatic nuclear transfer technique by using rabbits as the model. The oocyte recipients aged 16 h post coitus were collected surgically from 20 superovulated rabbit doe with 28 and 40 mg Follicle Stimulating Hormone (FSH) after mating with a vasectomized male. The metaphase II plate and 1st polar body of oocyte was later aspirated by enucleated micropipette under an inverted microscope. A single donor cell; cumulus cell or cultured or frozen fibroblast cell from passage 1 to 9 were transferred to enucleated oocyte and fused with triple DC pulses, 3.2 kv, 20 micros. The fused embryos were cultivated in TCM 199 NaHCO3 + 10 per cent fetal calf serum (FCS) for 4 days. The cleavage rate (2-cell stage) was 37.2 per cent (32/86) from eight experiments, and 18.8 per cent (6/32) developed to the early morula stage. This study also indicated that the enucleation pipette and the somatic cell type influenced the success.en_US
dc.description.affiliationDepartment of Obstetrics and Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.en_US
dc.identifier.citationTechakumphu M, Numchaisrika P, Suwajanakorn S, Pruksananonda K, Boonkasemsanti W, Ahnonkitpanit V, Virutamasen P. Preliminary study on somatic cell nuclear transfer in rabbits in Thailand. Journal of the Medical Association of Thailand. 2003 Mar; 86(3): 276-81en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/38301
dc.language.isoengen_US
dc.source.urihttps://www.mat.or.th/journal/all.phpen_US
dc.subject.meshActive Transport, Cell Nucleusen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCloning, Organism --methodsen_US
dc.subject.meshCoculture Techniquesen_US
dc.subject.meshEmbryonic and Fetal Development --physiologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshFibroblasts --cytologyen_US
dc.subject.meshMaleen_US
dc.subject.meshModels, Animalen_US
dc.subject.meshOocytes --growth & developmenten_US
dc.subject.meshRabbitsen_US
dc.subject.meshSensitivity and Specificityen_US
dc.titlePreliminary study on somatic cell nuclear transfer in rabbits in Thailand.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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