Comparison of potassium hydroxide mount and mycological culture with histopathologic examination using periodic acid-Schiff staining of the nail clippings in the diagnosis of onychomycosis.

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dc.contributor.authorShenoy, M Manjunathen_US
dc.contributor.authorTeerthanath, Sen_US
dc.contributor.authorKarnaker, Vimal Ken_US
dc.contributor.authorGirisha, B Sen_US
dc.contributor.authorKrishna Prasad, M Sen_US
dc.contributor.authorPinto, Jeromeen_US
dc.date.accessioned2009-05-28T08:17:13Z
dc.date.available2009-05-28T08:17:13Z
dc.date.issued2008-05-28en_US
dc.description.abstractBACKGROUND: Onychomycosis is a common problem noticed in clinical practice. Currently available standard laboratory methods show inconsistent sensitivity; hence there is a need for newer methods of detection. AIMS: This study involves comparison of standard laboratory tests in the diagnosis of onychomycosis, namely, potassium hydroxide mount (KOH mount) and mycological culture, with histopathologic examination using periodic acid-Schiff (PAS) staining of the nail clippings. METHODS: A total of 101 patients with clinically suspected onychomycosis were selected. Nail scrapings and clippings were subjected to KOH mount for direct microscopic examination, culture using Sabouraud's dextrose agar (with and without antibiotics) and histopathologic examination with PAS staining (HP/PAS). Statistical analysis was done by McNemar's test. RESULTS: Direct microscopy with KOH mount, mycological culture, and HP/PAS showed positive results in 54 (53%), 35 (35%), and 76 (75%) patients respectively. Laboratory evidence of fungal infection was obtained in 84 samples by at least one of these three methods. Using this as the denominator, HP/PAS had a sensitivity of 90%, which was significantly higher compared to that of KOH mount (64%) or mycological culture (42%). CONCLUSIONS: Histopathologic diagnosis with PAS staining of nail clippings was the most sensitive among the tests. It was easy to perform, rapid, and gave significantly higher rates of detection of onychomycosis compared to the standard methods, namely KOH mount and mycological culture.en_US
dc.description.affiliationDepartment of Dermatology, K. S. Hegde Medical Academy, Mangalore, Karnataka, India. manjunath576117@yahoo.co.inen_US
dc.identifier.citationShenoy MM, Teerthanath S, Karnaker VK, Girisha BS, Krishna Prasad MS, Pinto J. Comparison of potassium hydroxide mount and mycological culture with histopathologic examination using periodic acid-Schiff staining of the nail clippings in the diagnosis of onychomycosis. Indian Journal of Dermatology, Venereology and Leprology. 2008 May-Jun; 74(3): 226-9en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/52383
dc.language.isoengen_US
dc.source.urihttps://www.ijdvl.comen_US
dc.subject.meshAdolescenten_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAged, 80 and overen_US
dc.subject.meshArthrodermataceae --isolation & purificationen_US
dc.subject.meshCulture Mediaen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshHydroxidesen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshMitosporic Fungi --isolation & purificationen_US
dc.subject.meshMycology --methodsen_US
dc.subject.meshNails --microbiologyen_US
dc.subject.meshOnychomycosis --diagnosisen_US
dc.subject.meshPeriodic Acid-Schiff Reactionen_US
dc.subject.meshPotassium Compounds --diagnostic useen_US
dc.subject.meshSensitivity and Specificityen_US
dc.titleComparison of potassium hydroxide mount and mycological culture with histopathologic examination using periodic acid-Schiff staining of the nail clippings in the diagnosis of onychomycosis.en_US
dc.typeComparative Studyen_US
dc.typeEvaluation Studiesen_US
dc.typeJournal Articleen_US
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