Characterization of bacterial pathogens in Muga silkworm, Antheraea assamensis Helfer (Lepidoptera: Saturniidae)
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Date
2023-05
Journal Title
Journal ISSN
Volume Title
Publisher
Triveni Enterprises
Abstract
Aim: To identify and characterize the bacterial pathogens associated with bacteriosis in muga silkworm, A. assamensis. Methodology: The mid gut of diseased silkworm, A. assamensis was dissected and bacterial pathogens were cultured. Bacterial pathogens were identified by 16S rRNA gene sequencing. Insect bioassay studies were conducted to understand the pathogenicity of bacterial isolates. Results: Bacterial pathogens were identified as Pseudomonas aeruginosa and Bacillus mycoids. Under laboratory conditions, approximately 70% mortality of A. assamensis larvae was due to P. aeruginosa, while 50% larval mortality was due to B. mycoids. Interpretation: P. aeruginosa was relatively more pathogenic to A. assamensis than B. mycoids. Further, a graph on disease progression was developed to understand the trajectory of bacteriosis in A. assamensis.
Description
Keywords
Bacteriosis, Disease progression, Muga silkworm, Pseudomonas sp., 16S rRNA sequencing
Citation
Subrahmanyam G., Das R., Debnath R., Chutia M., Ponnuve K.M., Sathyanarayana K.. Characterization of bacterial pathogens in Muga silkworm, Antheraea assamensis Helfer (Lepidoptera: Saturniidae). Journal of Environmental Biology. 2023 May; 44(3): 479-484