A rapid HPTLC method to estimate piperine in Ayurvedic formulations

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dc.contributor.authorHazra, Alok K.en_US
dc.contributor.authorChakraborty, Bantien_US
dc.contributor.authorMitra, Achintyaen_US
dc.contributor.authorSur, Tapas Kumaren_US
dc.date.accessioned2020-11-18T10:11:56Z
dc.date.available2020-11-18T10:11:56Z
dc.date.issued2019-10
dc.description.abstractBackground: Trikatu, Sitopaladi, Hingavastaka, Avipattikara, Sringyadi and Talisadya are very popular Ayurvedic (churna) medicines practiced in India; however, unfortunately, they possess several qualitycontrol issues.Objective: The aim of this study was to find out a simple, accurate and sensitive HPTLC method for thedetection and quantification of marker molecule, piperine (alkaloid) on these Ayurvedic formulations forstandardization.Materials and methods: Methanolic extraction (reflux) was performed from the above six churnas as wellas three single ingredients Piper longum (pipul), Piper nigrum (marich) and Piper chaba (chai). HPTLC wasdone using piperine as a standard. The mobile phase was a mixture of toluene-ethyl acetate (7:3, v/v) anddetection at 342l.Results: The Rf was detected at 0.39. Piperine was quantified in all samples. P. nigrum showed higherpiperine than P. longum and P. chaba. The maximum piperine was noted in Hingavastaka churna andfollowed by Sringyadi churna, Sitopaladi churna, Talisadya churna, Trikatu churna and Avipattikara churna.Conclusion: This method can be successfully employed for standardization and quantitative analysis ofpiperine in Ayurvedic formulations (churnas) and also be helpful to clinicians and pharmacists to drawsignificant role of piperine present in all these samples.© 2017 Transdisciplinary University, Bangalore and World Ayurveda Foundation. Publishing Services byElsevier B.V. This is an open access article under the CC BY-NC-ND license (https://creativecommons.org/licenses/by-nc-nd/4.0/).en_US
dc.identifier.affiliationsDivision of Pharmaceutical Chemistry, R.K.M.A Quality Testing Laboratory, Kolkata, 700103, Indiaen_US
dc.identifier.affiliationsNational Research Institute of Ayurvedic Drug Development, CCRAS, Kolkata, 700091, Indiaen_US
dc.identifier.affiliationsNational Research Institute of Ayurvedic Drug Development, CCRAS, Kolkata, 700091, Indiaen_US
dc.identifier.affiliationsDepartment of Pharmacology, I.P.G.M.E & R, Kolkata, 700020, Indiaen_US
dc.identifier.citationHazra Alok K., Chakraborty Banti, Mitra Achintya, Sur Tapas Kumar. A rapid HPTLC method to estimate piperine in Ayurvedic formulations. Journal of Ayurveda and Integrative Medicine. 2019 Oct; 10(4): 248-254en_US
dc.identifier.issn0975-9476
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/214088
dc.languageenen_US
dc.publisherElsevieren_US
dc.relation.issuenumber4en_US
dc.relation.volume10en_US
dc.source.urihttps://dx.doi.org//10.1016/j.jaim.2017.07.006en_US
dc.subjectPiper Piperine Alkaloid HPTLC Ayurvedaen_US
dc.titleA rapid HPTLC method to estimate piperine in Ayurvedic formulationsen_US
dc.typeJournal Articleen_US
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