Downregulation of hsa_circ_0000936 sensitizes resistant glioma cells to temozolomide by sponging miR-1294

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dc.contributor.authorHua, Linen_US
dc.contributor.authorHuang, Lifaen_US
dc.contributor.authorZhang, Xinen_US
dc.contributor.authorFeng, Haokeen_US
dc.date.accessioned2020-11-18T10:14:17Z
dc.date.available2020-11-18T10:14:17Z
dc.date.issued2020-07
dc.description.abstractGlioma is one of the most aggressive forms of brain tumor and is hallmarked by high rate of mortality,metastasis and drug resistance. Herein, we explore the role of circular RNA (circRNA) hsa_circ_0000936 inthe resistance of glioma cells to temozolomide (TMZ). In this study, Relative changes in gene expression levelswere compared using qRT-PCR. The role of hsa_circ_0000936 was characterized by cell count kit -8 assay andflow cytometry. Luciferase reporter assay was carried out for target validation.We found that hsa_circ_0000936was upregulated in glioma tissues as compared to their adjacent normal tissues. Increased expression ofhsa_circ_0000936 was found in the glioma tissues of patients showing resistance to TMZ compared with thatof patients showing sensitivity to TMZ. The upregulation of hsa_circ_0000936 was also confirmed in TMZresistant glioma cells. miR-1294 was downregulated in TMZ-resistant glioma cells and identified as a directtarget of hsa_circ_0000936. Downregulation of hsa_circ_0000936 increased the sensitivity of TMZ-resistantglioma cells towards TMZ. Moreover, restoration of miR-1294 could abrogate the promoting effect ofhsa_circ_0000936 on TMZ resistance in TMZ-resistant glioma cells. In conclusion, downregulation ofhsa_circ_0000936 sensitizes TMZ-resistant glioma cells to TMZ by sponging miR-1294, suggesting thathsa_circ_0000936 may be a potential target for overcoming the resistance of glioma cells to TMZ.en_US
dc.identifier.affiliationsFirst Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang, Chinaen_US
dc.identifier.affiliationsFirst Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang, Chinaen_US
dc.identifier.affiliationsDepartment of Neurosurgery, Zhejiang Provincial Hospital of Traditional Chinese Medicine, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310006, Zhejiang, Chinaen_US
dc.identifier.affiliationsFirst Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang, Chinaen_US
dc.identifier.citationHua Lin, Huang Lifa, Zhang Xin, Feng Haoke. Downregulation of hsa_circ_0000936 sensitizes resistant glioma cells to temozolomide by sponging miR-1294. Journal of Biosciences. 2020 Jul; : 1-10en_US
dc.identifier.issn0250-5991
dc.identifier.issn0973-7138
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/214256
dc.languageenen_US
dc.publisherIndian Academy of Sciencesen_US
dc.relation.volume45en_US
dc.source.urihttps://dx.doi.org//10.1007/s12038-020-00072-zen_US
dc.subjectChemoresistanceen_US
dc.subjectcircular RNAen_US
dc.subjectgliomaen_US
dc.subjecthsa_circ_0000936en_US
dc.subjectmiR-1294en_US
dc.subjecttemozolomideen_US
dc.titleDownregulation of hsa_circ_0000936 sensitizes resistant glioma cells to temozolomide by sponging miR-1294en_US
dc.typeJournal Articleen_US
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