Human platelet releasates combined with polyglycolic acid scaffold promote chondrocyte differentiation and phenotypic maintenance.
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Date
2015-03
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Abstract
In the present study, we aimed to demonstrate the differentiating properties of platelet-rich plasma releasates (PRPr)
on human chondrocytes seeded on a polygtlycolic acid (PGA) 3D scaffold. Gene expression and biochemical analysis
were carried out to assess the improved quality of our PGA-based cartilage constructs supplemented with PRPr. We
observed that the use of PRPr as cell cultures supplementation to PGA-chondrocyte constructs may promote
chondrocyte differentiation, and thus may contribute to maintaining the chondrogenic phenotype longer than
conventional supplementation by increasing high levels of important chondrogenic markers (e.g. sox9, aggrecan
and type II collagen), without induction of type I collagen. Moreover, our constructs were analysed for the secretion
and deposition of important ECM molecules (sGAG, type II collagen, etc.). Our results indicate that PRPr supplementation
may synergize with PGA-based scaffolds to stimulate human articular chondrocyte differentiation, maturation
and phenotypic maintenance.
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Keywords
Chondrocyte, growth factors, PGA, platelet-rich plasma, tissue engineering
Citation
Bernardini Giulia, Chellini Federico, Frediani Bruno, Spreafico Adriano, Santucci Annalisa. Human platelet releasates combined with polyglycolic acid scaffold promote chondrocyte differentiation and phenotypic maintenance. Journal of Biosciences. 2015 Mar; 40 (1): 61-69.