Cloning, expression and characterization of aerolysin from Aeromonas hydrophila in Escherichia coli.

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dc.contributor.authorZhu, Dalingen_US
dc.contributor.authorLi, Aihuaen_US
dc.contributor.authorWang, Jianguoen_US
dc.contributor.authorLi, Mingen_US
dc.contributor.authorCai, Taozhenen_US
dc.date.accessioned2007-08-01en_US
dc.date.accessioned2009-05-27T09:36:01Z
dc.date.available2007-08-01en_US
dc.date.available2009-05-27T09:36:01Z
dc.date.issued2007-08-01en_US
dc.description.abstractAerolysin is a toxin (protein in nature) secreted by the strains of Aeromonas spp. and plays an important role in the virulence of Aeromonas strains. It has also found several applications such as for detection of glycosylphosphatidylinositol (GPI)-anchored proteins etc. A. hydrophila is a ubiquitous Gram-negative bacterium which causes frequent harm to the aquaculture. To obtain a significant amount of recombinant aerolysin in the active form, in this study, we expressed the aerolysin in E. coli under the control of T7 RNase promoter. The coding region (AerA-W) of the aerA gene of A. hydrophila XS91-4-1, excluding partial coding region of the signal peptide was cloned into the vector pET32a and then transformed into E. coli b121. After optimizing the expression conditions, the recombinant protein AerA-W was expressed in a soluble form and purified using His.Bind resin affinity chromatography. Recombinant aerolysin showed hemolytic activity in the agar diffusive hemolysis test. Western blot analysis demonstrated good antigenicity of the recombinant protein.en_US
dc.description.affiliationThe Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.en_US
dc.identifier.citationZhu D, Li A, Wang J, Li M, Cai T. Cloning, expression and characterization of aerolysin from Aeromonas hydrophila in Escherichia coli. Indian Journal of Biochemistry & Biophysics. 2007 Aug; 44(4): 204-8en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/27993
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshAeromonas hydrophila --metabolismen_US
dc.subject.meshBacterial Toxins --immunologyen_US
dc.subject.meshChromatography, Affinityen_US
dc.subject.meshCloning, Molecular --methodsen_US
dc.subject.meshEscherichia coli --geneticsen_US
dc.subject.meshPore Forming Cytotoxic Proteins --immunologyen_US
dc.subject.meshProtein Sorting Signals --geneticsen_US
dc.subject.meshRecombinant Proteins --isolation & purificationen_US
dc.titleCloning, expression and characterization of aerolysin from Aeromonas hydrophila in Escherichia coli.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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