Granulocyte-macrophage colony-stimulating factor production by T lymphocytes in Plasmodium berghei-infected mice.

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dc.contributor.authorOwhashi, Men_US
dc.contributor.authorUemura, Hen_US
dc.contributor.authorKanbara, Hen_US
dc.contributor.authorNawa, Yen_US
dc.date.accessioned2009-05-27T14:54:16Z
dc.date.available2009-05-27T14:54:16Z
dc.date.issued1997-12-10en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractThe production of granulocyte-macrophage colony-stimulating factor (GM-CSF) by lymphocytes was examined in murine malaria. When spleen cells or lymph node cells from P. berghei-infected mice were cultured in vitro with malaria antigen, the GM-CSF production correlated with the incubation time up to 72 hours. When lymphocytes obtained at various days after infection were cultured with the antigen, GM-CSF became detectable as early as 2 days after infection, reached a peak at day 9 and then rapidly decreased. Production of GM-CSF was antigen-specific, and related to the dose of antigen. Treatment of lymphocytes with anti-Thy-1.2 antibody and complement resulted in almost complete loss of GM-CSF-producing activity, while treatment with either anti-CD4 or anti-CD8 antibody and complement resulted in partial loss of GM-CSF-producing activity, indicating that both CD4+ and CD8+ T cells are involved in GM-CSF production in malaria. GM-CSF exhibits glycoprotein nature, and has an apparent molecular weight of 36,000. The molecular properties of this T-cell derived GM-CSF were compared with those of known lymphokine GM-CSF.en_US
dc.description.affiliationFaculty of Integrated Arts and Sciences, University of Tokushima, Japan. ohashi@ias.tokushima-u.ac.jpen_US
dc.identifier.citationOwhashi M, Uemura H, Kanbara H, Nawa Y. Granulocyte-macrophage colony-stimulating factor production by T lymphocytes in Plasmodium berghei-infected mice. The Southeast Asian Journal of Tropical Medicine and Public Health. 1997 Dec; 28(4): 757-63en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/31396
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/publication.htmen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntigens, Protozoan --metabolismen_US
dc.subject.meshChromatography, Affinityen_US
dc.subject.meshChromatography, High Pressure Liquiden_US
dc.subject.meshFemaleen_US
dc.subject.meshGranulocyte-Macrophage Colony-Stimulating Factor --biosynthesisen_US
dc.subject.meshMalaria --immunologyen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred C57BLen_US
dc.subject.meshPlasmodium berghei --immunologyen_US
dc.subject.meshT-Lymphocytes --immunologyen_US
dc.titleGranulocyte-macrophage colony-stimulating factor production by T lymphocytes in Plasmodium berghei-infected mice.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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