Evaluation of three methods to determine the antimicrobial susceptibility of Mycobacterium tuberculosis.

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dc.contributor.authorMuralidhar, Sumathien_US
dc.contributor.authorSrivastava, Lakshmien_US
dc.date.accessioned2004-11-14en_US
dc.date.accessioned2009-05-27T06:59:11Z
dc.date.available2004-11-14en_US
dc.date.available2009-05-27T06:59:11Z
dc.date.issued2004-11-14en_US
dc.description.abstractBACKGROUND & OBJECTIVES: Tuberculosis continues to be a major public health problem in India, especially with the emergence of drug resistance. A study was carried out to establish a rapid and accurate method of susceptibility testing for Mycobacterium tuberculosis using three methods viz., proportion method by agar dilution on Middlebrook 7H11 agar, proportion method using the conventional Lowenstein-Jensen (L-J) medium and E test strip method. METHODS: A total of seventy five clinical isolates from pulmonary and extrapulmonary sites were characterised and speciated by biochemical tests, growth and other standard parameters, and eight random isolates, also by polymerase chain reaction (PCR). Antimicrobial susceptibility of M.tuberculosis was performed by proportion method on L-J medium and Middlebrook 7H11 agar medium for isoniazide (INH), rifampicin (RIF), ethambutol (EMB), streptomycin (STM) and ciprofloxacin (CIP) using recommended critical concentrations. The two methods were compared with the E test method. RESULTS: The 75 M.tuberculosis strains were isolated from sputum (47), pus (23), aspirate fluid (2), skin tissue (2) and gastric aspirate (1). Of these 49 (65.3%) isolates were sensitive and one (1.3%) was resistant to all the five drugs tested and by all the three methods. Eleven (14.7%) isolates were resistant to INH alone by the three methods. The E test method detected one isolate resistant to INH and 2 to RIF which were missed by the other two methods. The results obtained by all the three methods compared well. INTERPRETATION & CONCLUSION: The three methods viz., proportion methods with L-J, Middlebrook 7H11 agar and the E test concurred fully in 57 isolates (76%). Association between L-J and Middlebrook 7H11 agar methods was 59 per cent. E test and the L-J methods did not differ significantly for all the drugs. The finding show that the E test method is superior to the other two methods in terms of simplicity of performance and the rapidity of results. Another advantage is that the MIC values can also be obtained simultaneously by this method.en_US
dc.description.affiliationDepartment of Microbiology, Vardhman Mahavir Medical College & Safdarjang Hospital, New Delhi, India. sumathi@macmail.comen_US
dc.identifier.citationMuralidhar S, Srivastava L. Evaluation of three methods to determine the antimicrobial susceptibility of Mycobacterium tuberculosis. Indian Journal of Medical Research. 2004 Nov; 120(5): 463-7en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/18805
dc.language.isoengen_US
dc.source.urihttps://icmr.nic.in/ijmr/ijmr.htmen_US
dc.subject.meshAntitubercular Agents --pharmacologyen_US
dc.subject.meshHumansen_US
dc.subject.meshIndia --epidemiologyen_US
dc.subject.meshMicrobial Sensitivity Tests --methodsen_US
dc.subject.meshMycobacterium tuberculosis --drug effectsen_US
dc.subject.meshReproducibility of Resultsen_US
dc.subject.meshTime Factorsen_US
dc.subject.meshTuberculosis --drug therapyen_US
dc.titleEvaluation of three methods to determine the antimicrobial susceptibility of Mycobacterium tuberculosis.en_US
dc.typeEvaluation Studiesen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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