Detection of anti-nuclear antibodies from filter paper blood clots using indirect immunoenzyme technique: preliminary experience and results.

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dc.contributor.authorChopra, Aen_US
dc.contributor.authorAnuradha, Ven_US
dc.contributor.authorEdmonds, Jen_US
dc.date.accessioned2000-05-29en_US
dc.date.accessioned2009-05-31T05:30:08Z
dc.date.available2000-05-29en_US
dc.date.available2009-05-31T05:30:08Z
dc.date.issued2000-05-29en_US
dc.description.abstractBACKGROUND: The facilities to detect antinuclear antibodies (ANA) patterns in patients with systemic rheumatic diseases/connective tissue disorders (CTD) using indirect immunofluorescence (IIF) technique (the gold standard) are sparse; the technique is technically difficult and expensive. A simpler technique, such as the indirect immunoenzyme (IIE) which uses light microscopy, ought to be evaluated for widespread use in our setting. OBJECTIVE: To study the feasibility and relevance of IIE in demonstrating ANA patterns, both from serum and filter paper blood clots (FPBC), in patients with CTD. METHODS: In this pilot study, ANA were detected from sera and FPBC of 21 patients with proven CTD using IIE; paired FPBC and serum samples were simultaneously collected in 10 patients. All samples, coded randomly, were tested by IIE and IIF, along with positive and negative controls. RESULTS: Using IIE, the results of the ANA patterns obtained from FPBC eluates and sera were similar; homogenous (SLE-6, PSS-1, RA-4), speckled (SLE-8, PSS-2, Overlap CTD-1) and centromere (PSS-1). Four SLE patients showed mixed pattern; sensitivity of IIE for lupus was hundred percent. On comparing the results with the serum IIF, the Kappa statistic of agreement was 1 (perfect) and 0.4 (fair) for FPBC-IIF and FPBC-IIE respectively; the results matched between serum IIF and FPBC-IIE in 8 of the 10 paired samples tested. CONCLUSIONS: IIE can demonstrate ANA both from sera and FPBC. This pilot study besides demonstrating positive trends for further probe also creates an awareness for such a feasible technique. However a larger sample size would be required to carry out its evaluation as an alternative to IIF and as a screening technique.en_US
dc.description.affiliationCentre for Rheumatic Diseases (CRD) and Inlaks Budhrani Hospital, Pune.en_US
dc.identifier.citationChopra A, Anuradha V, Edmonds J. Detection of anti-nuclear antibodies from filter paper blood clots using indirect immunoenzyme technique: preliminary experience and results. Journal of the Association of Physicians of India. 2000 May; 48(5): 493-6en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/95627
dc.language.isoengen_US
dc.source.urihttps://www.japi.orgen_US
dc.subject.meshAdulten_US
dc.subject.meshAntibodies, Antinuclear --blooden_US
dc.subject.meshArthritis, Rheumatoid --diagnosisen_US
dc.subject.meshBlood Specimen Collectionen_US
dc.subject.meshChilden_US
dc.subject.meshConnective Tissue Diseases --diagnosisen_US
dc.subject.meshFeasibility Studiesen_US
dc.subject.meshFemaleen_US
dc.subject.meshFluorescent Antibody Technique, Indirecten_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoenzyme Techniquesen_US
dc.subject.meshIndiaen_US
dc.subject.meshMaleen_US
dc.subject.meshMass Screeningen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshPilot Projectsen_US
dc.subject.meshPredictive Value of Testsen_US
dc.titleDetection of anti-nuclear antibodies from filter paper blood clots using indirect immunoenzyme technique: preliminary experience and results.en_US
dc.typeComparative Studyen_US
dc.typeEvaluation Studiesen_US
dc.typeJournal Articleen_US
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